Recombinant Human PRDX3 Protein

Name: Recombinant Human PRDX3 Protein
Brand: Beta LifeScience
SKU: BL-2061NP
Availability: InStock

BL-2061NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Peroxiredoxin-3 is produced by our E.coli expression system and the target gene encoding Pro63-Gln256 is expressed.
Accession	P30048
Synonym	Thioredoxin-Dependent Peroxide Reductase Mitochondrial; Antioxidant Protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog; PRDX3; AOP1
Gene Background	Thioredoxin-Dependent Peroxide Reductase Mitochondrial (PRDX3) is an enzyme that belongs to the AhpC/TSA family. Human and mouse PRDX3 genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Human PRDX3 protein has an antioxidant function and is localized in the mitochondrion. PRDX3 is involved in redox regulation of the cell. PRDX3 protects radical-sensitive enzymes from oxidative damage by a radical-generating system. It acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Molecular Mass	21.6 KDa
Apmol Mass	25 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Subcellular Location	Mitochondrion. Cytoplasm. Early endosome.
Protein Families	Peroxiredoxin family, AhpC/Prx1 subfamily
Database References	HGNC: 9354 OMIM: 604769 KEGG: hsa:10935 STRING: 9606.ENSP00000298510 UniGene: PMID: 28240739 results suggest that decreased PRDX3 by excessive bile acids in trophoblasts plays a critical role in the pathogenesis and progression of Intrahepatic cholestasis of pregnancy. PMID: 27958341 Mutants created to investigate link between oligomerisation and peroxidase activity. Obligate dimer is less active than wildtype but still displays peroxidase activity. 2.4A structure (PDB: 5UCX) shows cysteine-stabilised protein interface. PMID: 29438714 the present study identified the protein profiles of the PRDX family in LSCC and showed that down-regulation of PRDX3 in Hep-2 cells induced cell apoptosis and inhibited cell proliferation and cell migration. PMID: 27966448 The UNG1-PRDX3 interaction protected UNG1 from reactive oxygen species-mediated degradation and prevented mitochondrial DNA oxidation. PMID: 27480846 PRDX3 knockdown led to the down-regulation of ATP synthases and the decreased cellular ATP level, contributing to the slow-down of cell growth. Furthermore, silencing PRDX3 enhanced invasive properties of HepG2 cells via TIMP-1 down-regulation and the increased ECM degradation PMID: 26983019 Prx3 and Trx2 comprise an adaptive system to sense changes in atmospheric oxygen tension and influence cellular injury responses through both detoxification of mitochondrial oxidants and regulation of mitochondrial redox-dependent signaling PMID: 28045936 PRX3 plays a crucial role in mitochondrial homeostasis. PMID: 27037278 High PRX3 expression is associated with invasion of lung adenocarcinoma. PMID: 26810067 We also found that forkhead box protein 1 (FOXM1)-induced peroxiredoxin 3 (PRDX3) maintains the mitochondrial function, and the FOXM1/PRDX3 mitochondrial pathway maintains survival of colon CSCs PMID: 26350748 Oxidative stress promotes PRX2 and PRX3 hyperoxidation and attenuates pro-survival signaling in aging chondrocytes. PMID: 26797130 the species with one disulfide and one hyperoxidized active site was decameric for Prx2 and dimeric for Prx3. Reduction and re-oxidation of the hyperoxidized dimer of Prx3 produced hyperoxidized monomers PMID: 26614766 Data indicate that peroxiredoxin III (PrxIII) is a common direct target of both miroRNAs miR-26a-5p and miR-23b-3p. PMID: 24828865 Colon cancer stem cells overexpress the mitochondrial gene PRX3, which is required for maintenance of mitochondrial function and tumorigenesis, and is regulated by forkhead box protein 1, which also regulates expression of CD133 in these cells. PMID: 26091938 High serum PRDX3 levels are associated with hepatocellular carcinoma. PMID: 25743848 Peroxiredoxin 3 levels regulate a mitochondrial redox setpoint in malignant mesothelioma cells. PMID: 25462069 two placental proteins, Prx3 and Prx4, may act as new placental immune targets. PMID: 25323516 Serum PRDX3 can be used as a noninvasive biomarker for the diagnosis and/or prognosis of hepatocellular carcinoma. PMID: 24815434 High peroxiredoxin 3 protein expression is associated with hepatocellular carcinoma. PMID: 24105047 HIF-1alpha is a transcription factor for PRDX3 gene expression in clear cell renal cell carcinoma. PMID: 25093297 Data indicate that engineered Prx2 and Prx3 variants C-terminal residues modulate the extent of hyperoxidation. PMID: 24003226 The silencing of PRDX3 triggered cisplatinmediated apoptosis in the ovarian cancer cells, which may act through suppression of the NFkappaB signaling pathway. PMID: 23503975 Results suggest that PRDX-3 has an essential role in regulating oxidation-induced apoptosis in antiandrogen-resistant prostate cancer cells. PMID: 23880827 peroxiredoxin III may be associated with drug resistance in ovarian cancer PMID: 23564483 A fraction of FOXM1 coexists in the cytoplasm with mitochondrial peroxiredoxin 3. PMID: 23018647 Drug resistance formation was accompanied by a significant increase in the expression of PRDX1, PRDX2, PRDX3, PRDX6 genes in all cancer cell strains, which confirms the importance of redox-dependent mechanisms into development of cisplatin resistance. PMID: 23113308 Medulloblastoma tumors exhibit decreased expression of miR-383 but elevated expression of PRDX3. Up-regulation of miR-383 knocks down the expression of PRDX3, inhibits cell proliferation and promotes apoptosis. PMID: 23157748 the effects of PRDX3 in PCa progression may be caused by the regulation function of miR-23b, and consequently, miR-23b may be involved in the response of PCa cells to hypoxia stress PMID: 22710126 Single nucleotide polymorphisms in the PRDX3 and RPS19 is associated with HPV persistence and cervical precancer/cancer. PMID: 22496757 The results indicated that overexpression of PRDX3 was associated with 94.9% hepatocellular carcinoma, and correlated with poor differentiation (P<0.05), which suggest that PRDX3 has substantial clinical impact on the progression of hepatocarcinoma. PMID: 22344546 Report nuclear factor E2-related factor 2 dependent overexpression of sulfiredoxin and peroxiredoxin III in human lung cancer. PMID: 22016591 Cullin 4B protein ubiquitin ligase targets peroxiredoxin III for degradation. PMID: 21795677 The interaction between genetic variations in the PRDX3 gene and dietary fat intake is important for modulation of BMI and obesity risk. PMID: 21127481 Model for the exceptional reactivity of peroxiredoxins 2 and 3 with hydrogen peroxide: a kinetic and computational study. PMID: 21385867 Paraneoplastic autoimmune reaction against retinal pigment epithelium, with PRDX3 as the putative antigen, may be a cause of acute exudative polymorphous vitelliform maculopathy (AEPVM). PMID: 21220625 Both thioredoxin 2 and glutaredoxin 2 contribute to the reduction of the mitochondrial 2-Cys peroxiredoxin Prx3. PMID: 20929858 Prx3, which resides within mitochondria, were reacted with a selection of hydroperoxides generated by gamma-radiolysis or singlet oxygen, on free amino acids, peptides and proteins. PMID: 20840079 Peroxiredoxin III was significantly up-regulated in cervical cancer tissues. PMID: 20718351 Peroxiredoxin III has a significant role in cell cycle regulation and could be a potential proliferation marker in breast cancer. PMID: 20043069 Protein levels of Prx-III, a mitochondrial protein were significantly decreased in Alzheimer Disease and Down Syndrome. PMID: 11771746 Proteomics analysis of cellular response to oxidative stress. Evidence for in vivo overoxidation of peroxiredoxins at their active site PMID: 11904290 PRDX3 is a c-Myc target gene that is requried to maintain normal mitochondrial function. c-Myc directly activates expression of a mitochondrial peroxiredoxin that is required for Myc-mediated transformation. PMID: 12011429 LZK was associated directly with the IKK complex through the kinase domain, and that AOP-1 was recruited to the IKK complex through the binding to LZK. PMID: 12492477 The level of peroxiredoxin 3 is decreased in the frontal cortex of patients with Down syndrome and Pick's disease. PMID: 12650976 Peroxiredoxin III is a critical regulator of the abundance of mitochondrial H(2)O(2), which itself promotes apoptosis in cooperation with other mediators of apoptotic signaling PMID: 15280382 findings indicate that RPK118 is a PRDX3-binding protein that may be involved in transporting PRDX3 from the cytoplasm to its mitochondrial site of function or to other membrane structures via endosome trafficking. PMID: 15750338 The additive protection by Trx2 and GSH shows that Trx2 and GSH systems are both functionally important at low oxidative stress conditions. PMID: 17548047 Finally, the expression level of beta-globin and GATA-1 was dramatically increased in Prdx III O/E cell line. PMID: 17574212 Present throughout lens and localized to mitochondria in lens epithelial cells. Induction is acute response of lens to increased H2O2. Important role in lens H2O2-detoxification, mitochondrial maintenance, and possibly cataract formation. PMID: 17893648 FOXO3A mediates Prx III expression, and this may play a critical role in the resistance to oxidative stress in cardiac fibroblasts PMID: 18195003

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