Recombinant Human POR Protein

Name: Recombinant Human POR Protein
Brand: Beta LifeScience
SKU: BL-1577SG
Availability: InStock

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Tag	GST
Host Species	Human
Accession	BC034277
Synonym	CYPOR
Background	POR is a flavoprotein that donates electrons to all microsomal P450 enzymes, including the steroidogenic enzymes P450c17, P450c21, and CYP51A1. POR encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain which binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes (1). POR has been linked with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome (2).
Description	Recombinant full-length human POR was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source	Sf9 insect cells
AA Sequence	Full Length
Molecular Weight	~102 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human POR Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Subcellular Location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side.
Protein Families	NADPH--cytochrome P450 reductase family; Flavodoxin family; Flavoprotein pyridine nucleotide cytochrome reductase family
Database References	HGNC: 9208 OMIM: 124015 KEGG: hsa:5447 STRING: 9606.ENSP00000419970 UniGene: PMID: 29233819 genetic association studies in population in Scotland: data suggest, in type 2 diabetes treated with sulfonylureas, 2 SNPs in CYP2C9 (CYP2C92, R144C, rs1799853; CYP2C93, I359L, rs1057910) are associated with drug-induced hypoglycemia; an SNP in POR (POR28, A503V, rs1057868) is associated with better response to sulfonylureas. (CYP2C9 = cytochrome P450 family 2 subfamily C member 9; POR = cytochrome p450 oxidoreductase) PMID: 28656666 The POR28 heterozygote appears to be associated with the stable dose of acenocoumarol. PMID: 28592191 Younger age, POR28 allele, and CYP3A53 allele were associated with higher cyclosporine dosing requirements and lower concentration/dose ratio. PMID: 29135906 Suggest that hepatic POR and P450s are coregulated, and that POR plays a complex role in P450-mediated metabolism. PMID: 27271371 A homodimer model can resolve the conundrum as to how cytochrome P450 oxidoreductase and cytochrome b5 compete for the same binding site on cytochrome P450c17. (Review) PMID: 28000554 Compound heterozygosity of a paternal submicroscopic deletion and a maternal missense mutation in POR gene: Antley-bixler syndrome phenotype in three sibling fetuses PMID: 26969897 analysis of mutations and polymorphisms in POR linked to metabolism of steroids and xenobiotics and pathology of P450 oxidoreductase deficiency (PORD) PMID: 27068427 No significant differences in the distribution of haplotypes between cases and controls were detected. In conclusion, this study reveals that four SNPs in the POR gene (rs3823884, rs3898649, rs239953 and rs17685) may affect the susceptibility of smoking cessation in a Chinese Han population. PMID: 27660057 analysis of the role of the active site loop in coenzyme binding and flavin reduction in cytochrome P450 reductase PMID: 27461959 Current studies demonstrate that, unlike previously described mutations, A287P causes POR deficiency disorder due to conformational instability leading to proteolytic susceptibility in vivo, rather than through an inherent flavin-binding defect. PMID: 27496950 population pharmacokinetic analysis identified that the combined genotype of CYP3A5-POR was the only covariant for the apparent clearance of tacrolimus PMID: 26227094 Case Report: delayed diagnosis of 46, XY disorder of sex development due to P450 oxidoreductase (POR) deficiency. PMID: 27376429 Interindividual variability in CYP2C19 activity is due to differences in both CYP2C19 protein content associated with gene diplotypes and the cytochrome P450 oxidoreductase content. PMID: 26323597 POR rs2868177 and CYP2B66 variants contribute to the interindividual variability in human CYP2B6 activity, which may affect the disposition and interaction of other CYP2B6 substrate drugs PMID: 27439448 For the first time, the SNP POR37 831-35C>T was confirmed as a minor but statistically significant factor associated with inter-individual variation in warfarin maintenance dose in Han Chinese PMID: 27488389 POR28 does not significantly influence Tac pharmacokinetic parameters. PMID: 26829596 the POR g.6593A>G polymorphism significantly influences CYP2B6 activity PMID: 26580670 P450 (Cytochrome) Oxidoreductase Gene (POR) Common Variant (POR28) Significantly Alters CYP2C9 Activity in Swedish, But Not in Korean Healthy Subjects PMID: 26669712 POR A503V might decrease the risk of bladder cancer by reducing its metabolic activity. PMID: 26123203 POR besides CYP2B6 can influence cyclophosphamide metabolism PMID: 26544874 Study is a thorough structural, dynamic, and thermodynamic characterization of the soluble form of human microsomal CPR using small-angle x-ray scattering and nuclear magnetic resonance spectroscopy under various conditions PMID: 25809265 This study confirmed that CYP3A51 was associated with lower tacrolimus trough concentrations. POR28 was associated with decreased tacrolimus trough concentrations although the effect was small possibly through enhanced CYP3A4 enzyme activity. PMID: 26067485 electron transfer between mCPR and aromatase PMID: 26087061 POR is the main determinant of SN30000 sensitivity in 3 different genetic backgrounds. Head and neck squamous cell carcinomas showed heterogeneous POR expression. There may be a relationship between HPV status and hypoxia-activated prodrug sensitivity. PMID: 26297733 We identified seven unreported SNP genetic variations. The p.Pro384Leu variant exhibited reduced enzymatic activities compared with wild-type. PMID: 25712184 Results show that combining the CYP3A51, POR28 and CYP3A422 genotypes allows partial differentiation of early tacrolimus dose requirements and the time to reach therapeutic target concentrations after transplantation. PMID: 25322286 In children with FH, carriage of POR28 allele is associated with reduced effect of atorvastatin on TChol and LDLc and therefore identifies FH children that may require higher atorvastatin doses to achieve full therapeutic benefits PMID: 25521355 Transient kinetic dissection of the reaction of POR with NADPH and the reduction in cytochrome c by POR using stopped-flow techniques revealed defects in individual electron transfer steps mediated by A287P. PMID: 25728647 Interactions of HO-2 with CPR and BVR, were evaluated. PMID: 25196843 Data shows that POR28 allele was not associated with the lipid-lowering effect of atorvastatin. PMID: 24430948 results showed that the CYP2C19 and/or P450 oxidoreductase genotypes have an impact on the clearance of clobazam and/or N-desmethylclobazam PMID: 24345815 NADPH-cytochrome P450 reductase is regulated by all-trans retinoic acid and by 1,25-dihydroxyvitamin D3 in human acute myeloid leukemia cells. PMID: 24642534 The role of Cytochrome P450 reductase is to provide electrons to a redox partner, the interactions with cytochrome P450 2D6 (2D6) were investigated and a possible complex structure is suggested. PMID: 23832577 results show that FMO3 E158K and POR A503V polymorphisms are minor sources of nicotine metabolism variation, insufficient to appreciably alter smoking. PMID: 24448396 POR28 allele is an important source of CYP2C9 activity variability and combined with CYP2C9 gene poly-morphisms may explain individual variability in the effect of sulfonylureas. PMID: 24464600 Seventeen SNPs of CYP3A5, CYP3A4, COMT, IL-10 and POR were identified. PMID: 24465960 Candidate-gene study shows that polymorphisms in PPARalpha and POR might predispose patients being treated with tacrolimus to the development of NODAT after kidney transplantation PMID: 24113216 Findings regarding the interaction of NADPH-P450 reductase (NPR) with cellular stress response (CSR) indicated function of NPR in hypoxic response. PMID: 24491563 Domain motion is linked closely to the individual steps of the catalytic cycle of cytochrome P450 reductase. PMID: 23911089 although POR expression is a potential biomarker of sensitivity to some HAP, identification of other one-electron reductases responsible for HAP activation is needed for their rational clinical development. PMID: 24196959 Structural, enzymatic and clinical implications of the NADPH P450 oxidoreductase mutations linked to newly identified disorders in humans, now categorized as POR deficiency[review]. PMID: 23353702 POR genetic variants affect CYP2C9 activities. The impact of a POR variant on catalysis varies with the isoform of CYP2C9 and the assay substrate. PMID: 22547083 Six novel NADPH-P450 oxidoreductase missense variants in Ashkenazi and Moroccan Jewish populations were identified. PMID: 22462747 The data suggested that cytochrome P450 reductase plays a major role in reactive oxygen species generation by liver cells under the basal conditions, but a minimal rike during simulated in vitro ischemia-reperfusion injury. PMID: 21973081 NADPH cytochrome P450 reductase prevents this translocation and promotes oligomerization of HO-1. PMID: 22545110 Studies indicate that electron transfer in cytochrome P450 reductase is conformationally gated. PMID: 21762695 Clinical, biochemical, and genetic findings in a large PORD cohort. PMID: 22162478 POR28 C > T polymorphism increases 1-hydroxylation of midazolam after intravenous administration. The polymorphism is associated with increased hepatic, but not intestinal, CYP3A activity in healthy Chinese volunteers. PMID: 22177374 These data indicate that the POR28 SNP is associated with additional increases in early tacrolimus dose-requirements in patients carrying a CYP3A5*1 allele. PMID: 21770725

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