Recombinant Human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha (mutated N345 K) Protein

Name: Recombinant Human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha (mutated N345 K) Protein
Brand: Beta LifeScience
SKU: BLA-6967P
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Host Species	Human
Accession	P27986P42336
Synonym	GRB1 MGC142161 MGC142163 p110 alpha p85 p85 alpha Phosphatidylinositol 3 kinase 85 kDa regulatory subunit alpha Phosphatidylinositol 3 kinase associated p 85 alpha Phosphatidylinositol 3 kinase catalytic 110 KD alpha Phosphatidylinositol 3 kinase catalytic alpha polypeptide Phosphatidylinositol 3 kinase regulatory 1 Phosphatidylinositol 3 kinase regulatory subunit alpha Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform Phosphoinositide 3 kinase catalytic alpha polypeptide Phosphoinositide 3 kinase regulatory subunit 1 Phosphoinositide 3 kinase regulatory subunit 1 (alpha) PI3 kinase p110 subunit alpha PI3 kinase regulatory subunit alpha PI3 kinase subunit alpha PI3 kinase subunit p85 alpha PI3K PI3K alpha PI3K regulatory subunit alpha PIK3CA PIK3R1 PtdIns 3 kinase p110 PtdIns 3 kinase regulatory subunit alpha PtdIns 3 kinase regulatory subunit p85 alpha PtdIns 3 kinase subunit alpha PtdIns 3 kinase subunit p110 alpha
Description	Recombinant Human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha (mutated N345 K) Protein was expressed in Baculovirus infected Sf9 cells. It is a Full length protein
Source	Baculovirus infected Sf9 cells
AA Sequence	MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARP EEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPG SSKTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLY RTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPA AVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKL SQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNE RQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDT ADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSS VVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLH EYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQT QERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLK KQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGN ENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRE SSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQH TSLVQHNDSLNVTLAYPVYAQQRR
Molecular Weight	86 kDa including tags
Purity	>80% Densitometry.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	The specific activity of this protein was determined to be 18 nmol/min/mg.
Formulation	Liquid Solution
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.