Recombinant Human Phosphoinositide 3-Kinase Regulatory Subunit 5 (PIK3R5) Protein (His-SUMO)

Name: Recombinant Human Phosphoinositide 3-Kinase Regulatory Subunit 5 (PIK3R5) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-08768P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Phosphoinositide 3-Kinase Regulatory Subunit 5 (PIK3R5) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q8WYR1
Target Symbol	PIK3R5
Synonyms	5-bisphosphate 3-kinase regulatory subunit; F730038I15Rik; FOAP 2; FOAP2; OTTHUMP00000135339; p101; P101 PI3K; p101-PI3K; Phosphatidylinositol 4; Phosphatidylinositol 4,5 bisphosphate 3 kinase regulatory subunit; Phosphoinositide 3 kinase, regulatory subunit 5; Phosphoinositide 3 kinase, regulatory subunit 5, p101; Phosphoinositide 3 kinase, regulatory subunit, polypeptide p101; Phosphoinositide 3-kinase regulatory subunit 5; PI3 kinase p101 subunit; PI3 kinase regulatory subunit 5; PI3-kinase p101 subunit; PI3-kinase regulatory subunit 5; PI3R5_HUMAN; PIK3 R5; PIK3R5; Protein FOAP 2; Protein FOAP-2; PtdIns 3 kinase p101; PtdIns 3 kinase regulatory subunit; PtdIns-3-kinase p101; PtdIns-3-kinase regulatory subunit
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MDSGYVEDSEESSSEWPWRRGSQERRGHRRPGQKFIRIYKLFKSTSQLVLRRDSRSLEGSSDTALPLRRAGSLCSPLDEPVSPPSRAQRSRSLPQPKLGTQLPSWLLAPASRPQRRRPFLSGDEDPKASTLRVVVFGSDRISGKVARAYSNLRRLENNRPLLTRFFKLQFFYVPVKRSHGTSPGACPPPRSQTPSPPTDSPRHASPGELGTTPWEESTNDISHYLGMLDPWYERNVLGLMHLPPEVLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFAARPVLLQVYQTELTFITGEKTTEIFIHSLELGHSAATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKGAISGRSRWSNLEKVCTSVNLNKACRKQEELDSSMEALTLNLTEVVKRQNSKSKKGFNQISTSQIKVDKVQIIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSDLSSPPQTPPDLPAQAAPDLCSLLCLPIMTFSGALP
Expression Range	1-494aa
Protein Length	Full Length of Isoform 2
Mol. Weight	70.9kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein-mediated activation of PIK3CG.
Subcellular Location	Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein.
Database References	HGNC: 30035 OMIM: 611317 KEGG: hsa:23533 STRING: 9606.ENSP00000392812 UniGene: PMID: 26173259 PIK3R5 promoter hypermethylation is associated with oral squamous cell carcinoma. PMID: 25374236 expression and activities of PI3Kgamma are modified differently by p87 and p101 in vitro and in living cells, arguing for specific regulatory roles of the non-catalytic subunits in the differentiation of PI3Kgamma signaling pathways. PMID: 24014027 Our characterization of the PIK3R5 protein and findings suggest that it may play a role in the development of the cerebellum and vermis. PMID: 22065524 p101 has a role in membrane recruitment and activation of PI3K gamma PMID: 12507995 analysis of functional domains in the p101 regulatory subunit of phosphoinositide 3-kinase gamma PMID: 15611065 Overexpression of p101 activates PI3Kgamma signaling and is associated with T-cell lymphomas PMID: 17486067

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.