Recombinant Human Perilipin-1 (PLIN1) Protein (His)

Name: Recombinant Human Perilipin-1 (PLIN1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-08005P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Perilipin-1 (PLIN1) Protein (His) is produced by our Yeast expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	O60240
Target Symbol	PLIN1
Synonyms	Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin A; Perilipin; Perilipin-1; PerilipinA; PLIN; PLIN1; PLIN1_HUMAN
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	EPESEFRDIDNPPAEVERREAERRASGAPSAGPEPAPRLAQPRRSLRSAQSPGAPPGPGLEDEVATPAAPRPGFPAVPREKPKRRV
Expression Range	411-496aa
Protein Length	Partial
Mol. Weight	11.2kDa
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.
Subcellular Location	Endoplasmic reticulum. Lipid droplet.
Protein Families	Perilipin family
Database References	HGNC: 9076 OMIM: 170290 KEGG: hsa:5346 STRING: 9606.ENSP00000300055 UniGene: PMID: 29031005 Expression of UCP2 and PLIN1 genes influences the resting metabolic rate in obese individuals and could predict the weight loss after bariatric surgery. PMID: 27376365 The PLIN 6 polymorphism of the perilipin gene may influence the risk of obesity during adolescence. PMID: 28274232 Variability at the PLIN1 locus is associated with variability in weight loss. Moreover, eating late is related to lower weight-loss effectiveness among carriers of the AA genotype at the PLIN1 14995A>T variant. PMID: 27629052 The strong association of PLIN1, CFD and ADIPOQ genes with adipogenesis prompted authors to study the influence the bone health status as evaluated by quantitative ultrasound (QUS) bone densitometer in a North Indian cohort. Overall, ADIPOQ (rs1501299 and rs3774261) and combined cluster of PLIN1 rs2304796 and rs2304795) and CFD (rs1683563) demonstrated correlation. PMID: 28189761 Perilipin 1 expression increased with adipocytic differentiation of liposarcoma subtypes showing statistical significance. PMID: 27646609 Based on the PCR with mismatched primers PLIN1 polymorphisms could be identified effectively in Chinese Han population. PMID: 27468581 Conserved amphipathic helices mediate lipid droplet targeting of PLIN1, PLIN2, and PLIN3. PMID: 26742848 Skeletal muscle PLIN proteins likely play a role in the hydrolysis of triglycerides stored in lipid droplets and the passage of fatty acids to the mitochondria for oxidation. PMID: 25971423 The functional PLIN1 rs6496589 may influence the risk of central obesity through possible regulation of lipid storage. PMID: 25529448 After bariatric surgery-induced weight loss, PLIN1 gene/protein expression in SAT increased significantly. Findings suggest a positive functional interaction between PLIN1 & mitochondrial biogenesis-related genes in human adipose tissue. PMID: 24126816 Use of molecular docking software to design perilipin-1 inhibitors as antiobesity agents. PMID: 24610610 This plin1 variant binds and stabilizes ABHD5 expression but still fails to inhibit basal lipolysis as effectively as wild-type perilipin 1. PMID: 25114292 In long-term steatosis models in vitro, TIP47, MLDP, adipophilin, and finally perilipin were gradually induced PMID: 24269473 Independently and in an interactive manner, PLIN (and ENPP1) contribute to the risk of type 2 diabetes in a Taiwanese population. PMID: 23111648 Chinese adults with high waist circumference may have a high risk of diabetes, especially those with allele T in rs1052700 or with allele A in rs894160 of perilipin gene and those with perilipin genotype AA (rs894160) may have a high risk of obesity. PMID: 23517113 Although specific for invasive sebaceous carcinoma, perilipin expression was not helpful in distinguishing sebaceous carcinoma in situ from squamous cell carcinoma in situ with clear cell change. PMID: 23642680 Perilipin is almost exclusively expressed in white adipocytes, so the serious metabolic sequelae observed in patients suggest that primary defects in adipose tissue can lead to all the typical features seen in patients with the metabolic syndrome.[review] PMID: 23392103 PKC activation is required for TSH-stimulated perilipin phosphorylation and lipolysis in differentiated adipocytes. PMID: 22730012 Reduced mRNA and protein content of Plin and G0S2 and borderline increased ATGL protein in sc adipose tissue from poorly controlled type 2 diabetic subjects. PMID: 22535977 The morning cortisol increase as indicated by CAR correlated positively and significantly with hPER1 gene expression in older women suggesting that hPER1 expression increases in response to the morning cortisol increase in older women PMID: 22580060 genetic association studies in populations in US Midwest (MN, UT): When ratio of saturated fat to carbohydrate is high in diet, plasma insulin and insulin resistance are higher in women who are carriers of the minor allele in a PLIN1 SNP (rs894160). PMID: 21193293 genetic association studies in obese women in Spain (gene-diet interactions): Women on reducing diet who carry 11482(G>A) A allele have lower reduction in waist circumference and greater decrease in lipid oxidation rate than non-A allele carriers. PMID: 21392418 Identified two heterozygous frameshift mutations in the perilipin gene (PLIN1) in three families with partial lipodystrophy, severe dyslipidemia, and insulin-resistant diabetes; findings define a novel dominant form of inherited lipodystrophy. PMID: 21345103 a novel role for perilipin expression in adipose tissue metabolism and regulation of obesity and its metabolic complications PMID: 19797618 The present study aimed at comparing expression and subcellular distribution of perilipin and hormone-sensitive lipase in two abdominal adipose tissues of lean and obese women. PMID: 20017959 Expression patterns of perilipin and adipophilin in nonalcoholic fatty liver disease livers vary with the size of lipid droplets PMID: 20032580 This study found no incrased risk of obesity in Chinese Han adults with a single nucleotide polymorphism of perilipin. PMID: 20163070 Variation in PLIN may affect glucose and lipid metabolism in women both with and without ariation in PLIN may affect glucose and lipid metabolism in women both with and without polycystic ovary syndrome. PMID: 19782423 Perilipin could be a factor behind impaired lipolysis in insulin-resistants sconditions. PMID: 12802495 perilipin was elevated in obese subjects, perhaps as a compensatory mechanism to limit basal lipolysis. PMID: 15001633 Genetic variation at the perilipin (PLIN) locus is associated with obesity-related phenotypes in White women PMID: 15355432 study suggested a significant contribution of perilipin(PLIN) polymorphism 1243 to the elevated total cholesterol (TC) levels indicating that PLIN gene may be involved in human lipid metabolism PMID: 15601966 data support the hypothesis that the perilipin(PLIN) locus may be a significant genetic determinant for obesity risk in whites and that women are more sensitive to the genetic effects of perilipin than men PMID: 15601970 The association of increased obesity risk and structure of PLIN gene is studied; results support the role of the PLIN locus as an ethnically dependent modulator of obesity risk in humans. PMID: 15770500 We show the presence and induction of perilipin in atheroma. PMID: 15961705 PLIN11482A carriers were resistant to weight loss, suggesting that this polymorphism may predict outcome of BW reduction strategies based on low-energy diets. PMID: 15985482 Perilipin targets a novel pool of lipid droplets for lipolytic attack by hormone-sensitive lipase PMID: 16243839 Cooperation with other transcription factors may be differentially involved in selective transactivation of the perilipin gene by different peroxisome proliferator-activated receptor subtypes. PMID: 16567422 PLIN 11482G-->A/14995A-->T polymorphisms modulate the association between SFAs/carbohydrate in diet and insulin resistance in Asian women. PMID: 16732014 Genetic variations in the perilipin gene can affect weight gain associated with rosiglitazone treatment in patients with type 2 diabetes. PMID: 16732015 Results suggest that that PLIN constitutes a susceptibility locus for reduced BMD in Japanese men. PMID: 16786163 The rs4578621 and rs894160 polymorphisms of the perilipin gene are not major genetic determinants of obesity and type 2 diabetes-related phenotypes in a random sample of French men and women. PMID: 16836753 PLIN is a candidate gene for obesity risk in humans as well as a modulator of dietary response to therapies aimed to reduce body weight and decrease metabolic syndrome risk. [REVIEW] PMID: 17353663 Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition PMID: 17927964 negative finding that the common variants of PLIN do not have a major effect on susceptibility to stroke in a Chinese population PMID: 18174481 The results of these studies demonstrated that A. phagocytophilum modulates lipid metabolism by increasing PLIN mRNA levels and facilitates infection of HL-60 cells. PMID: 18201980 Thus, the perilipin gene expression is regulated by a transcriptional network controlling energy metabolism, substantiating the functional importance of perilipin in the maintenance of body energy balance. PMID: 18243128 These 2 studies suggest that the presence of the minor C and A alleles at PLIN1 and PLIN4, respectively, are associated with a lower postprandial response that may result in lower atherogenic risk for these persons PMID: 18326614 Central obesity may modify the associations between PLIN variations and diabetes risk in women. PMID: 18356850

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human Perilipin-1 (PLIN1) Protein (His)

Recombinant Human Perilipin-1 (PLIN1) Protein (His)

Product Overview

Target Details

FAQs