Recombinant Human PDE5A Protein

Beta LifeScience SKU/CAT #: BL-0186SG
Recombinant Human PDE5A Protein

Recombinant Human PDE5A Protein

Beta LifeScience SKU/CAT #: BL-0186SG
Catalog No.: BL-0186SG

Product Overview

Tag GST
Host Species Human
Accession NM_001083
Synonym CN5A; PDE5; PDE5A1; CGB-PDE
Background PDE5A is a member of the cyclic nucleotide phosphodiesterase family. PDE5A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. PDE5A regulates nitric oxide (NO)-induced relaxation and cGMP breakdown in smooth muscle cells in vas deferens (1). PDE5A expression predisposes mice to adverse left ventricular remodeling after myocardial infarction. Furthermore, increased myocardial PDE5A expression in patients with advanced cardiomyopathy can contribute to the development of heart failure (2).
Description Recombinant human PDE5A (537-end) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source Sf9 insect cells
AA Sequence 537a.a.-end
Molecular Weight ~63 kDa
Purity For specific purity information on a given lot, see related COA.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Active
Formulation Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability The recombinant protein is stable for up to 12 months at -70°C
Usage For Research Use Only
Storage Recombinant Human PDE5A Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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