Recombinant Human PARP3 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3653
Recombinant Human PARP3 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3653
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His&GST |
Host Species | Human |
Accession | Q9Y6F1 |
Synonym | ADPRT3, ADPRTL2, ADPRTL3, ARTD3, IRT1, PADPRT-3 |
Background | Poly(ADP-ribose) polymerase 3 (PARP3) is an important member of the PARP family and shares high structural similarities with both PARP1 and PARP2. Poly(ADP-ribose) polymerase 3 (PARP3), a critical player in cellular response to DNA double-strand breaks (DSBs), plays an essential role in the maintenance of genome integrity. The ADP ribosyl transferase [poly(ADP-ribose) polymerase] ARTD3(PARP3) is a newly characterized member of the ARTD(PARP) family that catalyzes the reaction of ADP ribosylation, a key posttranslational modification of proteins involved in different signaling pathways from DNA damage to energy metabolism and organismal memory. |
Description | A DNA sequence encoding the C-terminal segment of human PARP3 isoform 1 (Q9Y6F1-1) (Met 1-Leu 533) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | Met |
AA Sequence | Met 1-Leu 533 |
Molecular Weight | The recombinant human PARP3/GST chimera consists of 768 a.a. and has a calculated molecular mass of 87.8 kDa. It migrates as an approximately 95 kDa band in SDS-PAGE under reducing conditions. |
Purity | >85% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |