Recombinant Human Nucleolar Gtp-Binding Protein 2 (GNL2) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09839P

Greater than 90% as determined by SDS-PAGE.
Recombinant Human Nucleolar Gtp-Binding Protein 2 (GNL2) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09839P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human Nucleolar Gtp-Binding Protein 2 (GNL2) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | Q13823 |
Target Symbol | GNL2 |
Synonyms | Autoantigen NGP-1; Autoantigen NGP1; DJ423B22.6 (novel nucleolar guanosine 5'-triphosphate binding protein; FLJ40906; GNL2; Guanine nucleotide binding protein-like 2 (nucleolar); HUMAUANTIG; NGP1; NOG2_HUMAN; novel nucleolar guanosine 5'-triphosphate binding protein autoantigen; nucleolar G-protein gene 1; Nucleolar GTP binding protein 2; Nucleolar GTP-binding protein 2; Nucleolar GTPase |
Species | Homo sapiens (Human) |
Expression System | E.coli |
Tag | N-6His-SUMO |
Target Protein Sequence | MVKPKYKGRSTINPSKASTNPDRVQGAGGQNMRDRATIRRLNMYRQKERRNSRGKIIKPLQYQSTVASGTVARVEPNIKWFGNTRVIKQSSLQKFQEEMDTVMKDPYKVVMKQSKLPMSLLHDRIRPHNLKVHILDTESFETTFGPKSQRKRPNLFASDMQSLIENAEMSTESYDQGKDRDLVTEDTGVRNEAQEEIYKKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRSPHIETYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASLTNPFGKGAFIQLLRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQYITLMRRIFLIDCPGVVYPSEDSETDIVLKGVVQVEKIKSPEDHIGAVLERAKPEYISKTYKIDSWENAEDFLEKLAFRTGKLLKGGEPDLQTVGKMVLNDWQRGRIPFFVKPPNAEPLVAPQLLPSSSLEVVPEAAQNNPGEEVTETAGEGSESIIKEETEENSHCDANTEMQQILTRVRQNFGKINVVPQFSGDDLVPVEVSDLEEELESFSDEEEEEQEQQRDDAEESSSEPEEENVGNDTKAVIKALDEKIAKYQKFLDKAKAKKFSAVRISKGLSEKIFAKPEEQRKTLEEDVDDRAPSKKGKKRKAQREEEQEHSNKAPRALTSKERRRAVRQQRPKKVGVRYYETHNVKNRNRNKKKTNDSEGQKHKRKKFRQKQ |
Expression Range | 1-731aa |
Protein Length | Full Length |
Mol. Weight | 99.7kDa |
Research Area | Epigenetics And Nuclear Signaling |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation. May promote cell proliferation possibly by increasing p53/TP53 protein levels, and consequently those of its downstream product CDKN1A/p21, and decreasing RPL23A protein levels. |
Subcellular Location | Nucleus, nucleolus. |
Protein Families | TRAFAC class YlqF/YawG GTPase family, NOG2 subfamily |
Database References | |
Tissue Specificity | Widely expressed, with the highest expression level in testis. |
Gene Functions References
- NGP-1 promotes cell cycle progression through the activation of the p53/p21(Cip-1/Waf1) pathway. PMID: 26203195
- GNL2 and SEC13 are required for nonsense-mediated mRNA decay pathway in human cells. PMID: 25452588