Recombinant Human NT-3 Protein

Name: Recombinant Human NT-3 Protein
Brand: Beta LifeScience
SKU: BL-1758NP
Availability: InStock

BL-1758NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality cytokines for advanced research, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Neurotrophin-3 is produced by our E.coli expression system and the target gene encoding Tyr139-Thr257 is expressed.
Accession	P20783
Synonym	Neurotrophin-3; NT-3; HDNF; Nerve Growth Factor 2; NGF-2; Neurotrophic Factor; NTF3
Gene Background	Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β-NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequences of mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems.
Molecular Mass	13.6 KDa
Apmol Mass	14 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
Endotoxin	Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Seems to promote the survival of visceral and proprioceptive sensory neurons.
Subcellular Location	Secreted.
Protein Families	NGF-beta family
Database References	HGNC: 8023 OMIM: 162660 KEGG: hsa:4908 STRING: 9606.ENSP00000397297 UniGene: PMID: 29549646 brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT3) levels in post-mortem brain tissue from patients with depression compared to healthy individuals - a proof of concept study PMID: 29102768 NT-3 has been shown to be both an osteogenic and angiogenic factor, and can also enhance expression of the key osteogenic factor, BMP-2, as well as the major angiogenic factor, VEGF, to promote bone formation, vascularization, and bone healing PMID: 28370021 High expression of NT-3 is associated with glioblastoma. PMID: 28402394 Meta-analysis found the levels of both NT-3 and NT-4/5 were significantly increased in bipolar disorder patients. Through subgroup analysis, this increase persisted only in patients in depressed state, but not in manic or euthymic state. In addition, we found the differences in NT-3 and NT-4/5 were significantly associated with the duration of illness, but not by the mean age or female proportion. PMID: 27214525 NT3 upregulates cellular proliferation, extracellular matrix protein production, and collagen deposition in human aortic valve interstitial cells through the Trk-Akt-cyclin D1 cascade. PMID: 28356268 The analysis of covariance (ANCOVA) indicated that the mean serum GDNF and NTF3 levels of ADHD patients were significantly higher than that of controls. However, serum BDNF and NGF levels did not show any significant differences between groups. These results suggest that elevated serum GDNF and NTF3 levels may be related to ADHD in children. PMID: 27561780 There were no differences in neurotrophin levels between patients with schizophrenia and controls. We found lower BDNF and higher NT-3 serum levels in depressed patients with schizophrenia. PMID: 26548545 The results suggest a gene dose-association between the A allele of rs6332 and the onset of AD in varepsilon4 non-carriers and the NTF-3 rs6489630 polymorphism being a relevant risk factor for AD in patients lacking the ApoE-varepsilon4 allele in this Chinese sample. PMID: 26814132 Survival, differentiation, and neuroprotective mechanisms of human stem cells complexed with neurotrophin-3-releasing pharmacologically active microcarriers in an ex vivo model of Parkinson's disease. PMID: 25925835 No differences in plasma BDNF, NGF, NT3, NT4 and GDNF were found between autism spectrum disorders and control. PMID: 25275256 NT-3 expression was found in axons of olfactory bulb. PMID: 24288162 genetic variation in the NTF3 gene is related to susceptibility to emotional side effects in response to methylphenidate treatment in Korean children with ADHD. PMID: 23471121 NT-3 appears to promote growth of metastatic breast cancer cells in the brain PMID: 23001042 NT-3 promoted motility, migration, invasion, soft-agar colony growth and cytoskeleton restructuring in TrkC-expressing U2OS cells PMID: 23027130 There was no association between the presence of rs11063714 and curve progression in adolescent idiopathic scoliosis in a 2117-person Japanese cohort. PMID: 23038618 Results suggested that an NT-3 polymorphism, rs6332, may significantly influence executive function, reflecting interference performances among patients with mild-stage AD. PMID: 23075484 These findings suggest that NT-3, BDNF, LIF and their combinations predominantly support different ontogenetic events at different developmental stages in the innervation of the inner ear. PMID: 23149719 we identify a TrkB ligand, neurotrophin 3 (NTF3), as capable of activating TrkB to induce anoikis resistance, and show that NTF3 is also a direct target of miR-200c PMID: 23185507 The specific aspects of proprioceptive sensory neuron subtype character is controlled by variation in the level of muscle NT-3 expression. PMID: 23522042 NTF3 is transported from midbrain dopaminergic neurons and supports the survival of immature medium-sized spiny neurons. PMID: 23426664 There is no significant difference in NGF, NT-3 and p75NTR expression in the myometrium or endometrium between the adenomyosis and the control group. PMID: 22454143 protein levels of translational, splicing, processing, chaperone, protein handling, and metabolism machineries were shown to depend on neurotrophin-3-induced TrkC activation in the medulloblastoma cell line DAOY PMID: 18336001 Mice carrying one or two platelet-derived growth factor beta-NT3 transgenes on a background null for wildtype NT-3 are generated by crossing with an NT-3 null strain; although still ataxic, mice from this cross could survive for periods longer than a year. PMID: 21787840 NTF3 gene polymorphisms are not associated with the occurrence of idiopathic scoliosis, but the promoter polymorphism (rs11063714) is associated with the curve severity, implicating an alleviating role of NTF3 in the curve progression. PMID: 22158057 Implantation of NT-3 gene-modified mesenchymal stem cells via a recombinant adenoviral vector into a demyelinated region of rat spinal cord results in significant improvement of locomotor function and electrophysiological restoration in rats. PMID: 21996274 the study demonstrates for the first time that a neurotrophin factor can synergize with IGFBP-2 to promote hematopoietic cell expansion PMID: 22459634 both apoptotic cell death and neuronal differentiation of tumor cells were the mechanisms of growth-inhibitory effect of NT-3-secreting human adipose tissue-derived mesenchymal stem cells PMID: 21720807 Release of peripheral blood mononuclear cell-derived NT-3 correlates with its concentration in the brain-parenchymal fraction and corpus callosum cross-sectional area and may exert a direct or indirect neuroprotective effect in multiple sclerosis. PMID: 22036954 findings suggest that endogenous CNTF and exogenous BDNF and NT-3 play roles in the differentiation of embryonic spinal cord derived progenitor cells into astrocytes, neurons and oligodendrocytes, respectively PMID: 21698095 Collagen-binding neurotrophin-3 promotes axonal regeneration after spinal cord transection. PMID: 20597688 Co-culture of human neurotrophin-3 (NT-3) gene-modified Schwann cells (SCs) and human NT-3 receptor tyrosine protein kinase C (TrkC) gene-modified MSCs increase differentiation of neuron-like cells from mesenchymal stem cells. PMID: 19680743 NT3 may be involved in early folliculogenesis, particularly in the activation of primordial follicles. PMID: 21392742 Results suggest that proneurotrophin-3 and proBDNF may play important roles in the response to noise-induced injuries or ototoxic damage via the Sortilin:p75(NTR) death-signalling complex. PMID: 21261755 Data show that serum NT-3 levels were similar in SSc and in the control group. PMID: 21085492 preliminary evidence of an association between NTF3 and the intelligence and selective attention deficit in the Korean population. PMID: 20576502 The results of this study suggested that increased serum NT-3 levels in BD are likely to be associated with the pathophysiology of manic and depressive symptoms. PMID: 20060128 Report the proangiogenic capacity of NT-3 and propose NT-3 as a novel potential agent for the treatment of ischemic disease. PMID: 20360537 TrkC ligand neurotrophin-3 (NT-3) is upregulated in a large fraction of aggressive human neuroblastomas (NBs) and that it blocks TrkC-induced apoptosis of human NB cell lines, consistent with the idea that TrkC is a dependence receptor. PMID: 20160348 NGF and NT-3 concentrations are increased in children with hydrocephalus. PMID: 11580868 Human eosinophils produce neurotrophin 3 on immunologic stimuli. PMID: 11877300 Results suggest that the G(- 3004)-A3 haplotype has a modest effect of giving susceptibility to schizophrenia. PMID: 11920853 NT3 is significantly decreased in the myenteric plexus of patients with active Crohn's disease and ulcerative colitis. PMID: 12598727 Fibroblasts genetically modified to express human neurotrophin-3 (NT-3) were placed in, and distal to, the lesion cavity in chronic spinal cord injured rats; grafted animals exhibited significant growth of corticospinal axons PMID: 12710933 Monocytes, produce, store and release nerve growth factor, brain-derived neurotrophic factor and NT-3 PMID: 15544837 In transgenic mlc/NT-3 mice following nerve injury, NT-3 plays an important role during the early stages of spindle denervation that ultimately effects reinnervation by group II and gamma fibers. PMID: 15589528 Circulating neurotrophin-3 levels increased in early neonatal life, possibly due to exposure to various stimuli soon after birth PMID: 15770067 The present data on neurotrophin-specific transcriptional down-regulation of NT3 in human Chronic obstructive pulmonary disease (COPD)indicate a pathophysiological role for neurotrophins in COPD. PMID: 15843147 Human bronchial smooth muscle cells can express NGF, BDNF and NT-3. Expression may be differently regulated by inflammatory cytokines. Might have potential role in airway inflammation. PMID: 16441896 Light and electron microscopy immunohistochemistry showed that tonsillar samples were positive for NT3. PMID: 16786155

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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