Recombinant Human NEGR1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3456
Recombinant Human NEGR1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3456
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_776169.2 |
Synonym | DMML2433, IGLON4, KILON, Ntra |
Background | Neuronal Growth Regulator 1, NEGR1, also known as neurotractin, or KILON, which belongs to the immunoglobulin superfamily, IgLON family. This GPI-linked cell surface glycoprotein NEGR1 is composed of three Ig-like domains and belongs to the IgLON subgroup of neural IgSF members. It is expressed in two isoforms with apparent molecular masses of 5 and 37 kD, termed L-form and S-form, respectively. NEGR1/Neurotractin participates in the regulation of neurite outgrowth in the developing brain, and is expressed on neurites of primary hippocampal neurons. Neurotractin/KILON is a trans-neural growth-promoting factor for outgrowing axons following hippocampal denervation. KILON (kindred of IgLON) and opioid-binding cell adhesion molecule belong to the IgLON subgroup of immunoglobulin superfamily together with the limbic system-associated membrane protein and neurotrimin. The alteration of modulatory function of KILON/NEGR1 for the number of dendritic synapses concomitant with changes in its localization and detergent solubility during neuronal culture development. In addition to its reported role in the brain, NEGR1 is also expressed in subcutaneous adipose tissue and acts as a central 'hub' in an obesity-related transcript network. |
Description | A DNA sequence encoding the human NEGR1 (NP_776169.2) (Met 1-Gly 324) without the propeptide was expressed, with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Val 38 |
AA Sequence | Met 1-Gly 324 |
Molecular Weight | The secreted mature form of recombinant human NEGR1 consists of 298 a.a. and predictes a molecular mass of 32.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NEGR1 is approximately 45-48 kDa due to glycosylation. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |