Recombinant Human Multidrug Resistance-Associated Protein 1 (ABCC1) Protein (His)

Name: Recombinant Human Multidrug Resistance-Associated Protein 1 (ABCC1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-02172P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Multidrug Resistance-Associated Protein 1 (ABCC1) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P33527
Target Symbol	ABCC1
Synonyms	ABC 29; ABC29; ABCC 1; ABCC; Abcc1; ATP binding cassette sub family C (CFTR/MRP) member 1; ATP binding cassette sub-family C member 1; ATP binding cassette subfamily C member 1; ATP binding cassette transporter variant ABCC1delta ex13; ATP binding cassette transporter variant ABCC1delta ex13&14; ATP binding cassette transporter variant ABCC1delta ex25; ATP binding cassette transporter variant ABCC1delta ex25&26; ATP binding cassette, sub-family C (CFTR/MRP), member 1; ATP-binding cassette sub-family C member 1; DKFZp686N04233; DKFZp781G125; GS X; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP 1; MRP; MRP1; MRP1_HUMAN; Multidrug resistance associated protein 1; Multidrug resistance protein; Multidrug resistance-associated protein 1; Multiple drug resistance associated protein; Multiple drug resistance protein 1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	VRMSSEMETNIVAVERLKEYSETEKEAPWQIQETAPPSSWPQVGRVEFRNYCLRYREDLDFVLRHINVTINGGEKVGIVGRTGAGKSSLTLGLFRINESAEGEIIIDGINIAKIGLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKDFVSALPDKLDHECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQSTIRTQFEDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEYGAPSDLLQQRGLFYSMA kDaGLV
Expression Range	1248-1531aa
Protein Length	Partial
Mol. Weight	35.9 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency. Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation. Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	ABC transporter superfamily, ABCC family, Conjugate transporter (TC 3.A.1.208) subfamily
Database References	HGNC: 51 OMIM: 158343 KEGG: hsa:4363 STRING: 9606.ENSP00000382342 UniGene: PMID: 30132569 Cysteine residues were introduced into TMs 8, 14, 15, and 16 of 3Cys DeltaMRP1. PMID: 29572332 Efflux of different arsenic metabolites by MRP1 is likely influenced by multiple factors. PMID: 29752257 Peripheral blood MRP1 expression is increased in patients with refractory epilepsy PMID: 26099600 Due to the downregulation of MDR1 and MRP1, the intracellular accumulation of ADM was increased in the transfected cells compared with that in the parental K562/ADM cells..Our research revealed that protein expression of the ERK signaling pathway was inhibited by downregulating TRIB2, indicating that the ERK pathway was involved in cell drug resistance and proliferation. PMID: 29436678 icaritin (ICT) decreased the mRNA and protein levels of multidrug resistance-associated protein 1 (MRP1) in MG-63 doxorubicin-resistant (MG-63/DOX) cells. PMID: 29425587 Enhanced BRAF-mediated NRF2 gene transcription and Histone Acetyltransferases-mediated NRF2 protein acetylation contributes to ABCC1-mediated chemoresistance and glutathione-mediated survival in acquired topoisomerase II poison-resistant cancer cells. PMID: 29080842 Pomolic acid also down-modulated the activity of the multidrug resistance associated protein 1 (MRP1) and inhibited migration of Glioblastoma (GBM) cells. These results show that PA acts on several pathways of GBM drug resistance and therefore may be of potential interest for the treatment of this tumor PMID: 28849227 Our data present for the very first time the high negative correlation between MRP1 protein expression in LSC and AML patients' remission. It does strongly suggest that MRP1 expression in LSC is an adverse prognostic marker in patients with de novo AML. PMID: 28805931 No significant correlation was observed between rs504348 genotypes and cystic fibrosis severity. A significantly higher expression of ABCC1 mRNA was induced by CF plasma compared to healthy control plasma. PMID: 28800122 MRP1 is regulated by CLIC1, leading to CLIC1-mediated development of chemoresistance in choriocarcinoma cells. PMID: 27983917 High MRP1 expression is associated with resistance to doxorubicin in breast cancer. PMID: 27878697 Our data indicates that hypomethylation of the ABCC1 promoter is not cancer type-specific but occurs in cancer cell lines of different origins. PMID: 27689338 Our work demonstrates for the first time that the adaptation to endoplasmic reticulum(ER) stress in cancer cells produces a MDR phenotype. The PERK/Nrf2/MRP1 axis is responsible for the resistance to ER stress and chemotherapy, and may represent a good therapeutic target in aggressive and resistant tumors. PMID: 28499449 Data show that miR-145 level decreased in breast cancer tissues, breast cancer cell lines and doxorubicin resistant MCF-7 cells, and inversely correlated with multidrug resistance-associated protein 1 (MRP1) expression level. PMID: 27487127 PG accumulation assay is a new, unique tool for the parallel determination of the function of the ABCG2, ABCB1, and ABCC1 multidrug transporters PMID: 29342177 ABCC1 exports corticosterone but not cortisol. We show that ABCC1 is expressed in adipose tissue and that ABCC1 inhibition increases intracellular corticosterone, but not cortisol, and induces glucocorticoid-responsive gene transcription in adipocytes. PMID: 27535620 GSK1904529A did not alter the expression level of MRP1, induced a G0/G1 phase cell cycle arrest. Our results indicated that GSK1904529A significantly increased the sensitivity of MRP1 overexpressing cells to chemotherapeutic agents. PMID: 28266043 High MRP1 expression is associated with ovarian cancer. PMID: 27739315 These results show that multidrug resistant leukemia cell microparticle regulation of ABCC1 in recipient cells is governed by the transfer of both miR-326 and ABCB1 from donor cells PMID: 28050891 Higher expression of ABCG2 and MRP1, and lower expression of CK19 in papillary thyroid carcinomas with a solid component were associated with higher tumor recurrence rate and shorter disease-free survival. PMID: 28411177 Visfatin increases the nuclear translocation of Akt and its binding with ABCC1. These data showed that visfatin can decrease doxorubicin sensitivity of Non-small-cell lung cancer cells via activation of Akt/MRP1. PMID: 28762597 Studied hedgehog signaling effect thru ATP-binding cassette (ABC) transporters in hepatoma drug sensitivity. Results suggest Hedgehog signaling transcription factor Gli-2 to be the primary regulator for drug sensitivity of hepatoma through the ABCC1 transporter. PMID: 28414325 This study demonstrated that mitochondrial MRP-1 is expressed in the outer mitochondrial membrane and is a client protein of HSP90beta. PMID: 26722004 High ABCC1 expression is associated with hepatic fibrosis. PMID: 28098912 This study shows direct binding between MRP1 Linker 1 (L1MRP1) domain and alpha- and beta-tubulin subunits, using a scanning peptide approach PMID: 27908733 In summary, chaetominine strongly reverses drug resistance by interfering with the PI3K/Akt/Nrf2 signaling, resulting in reduction of MRP1-mediated drug efflux and induction of Bax/Bcl-2-dependent apoptosis in an ADR-resistant K562/Adr leukemia cell line. PMID: 27038543 cross-talk between MRP1 glycosylation and phosphorylation occurs. PMID: 27297967 meta-analysis results strongly suggest that MRP1 is overexpressed in both neurons and astrocytes of intractable epilepsy patients, inhibition of MRP1 may enhance antiepileptic drug efficacy by increasing local drug availability PMID: 26000815 Our results show MRP1 as a potential biomarker of resistance to treatment with irinotecan when found in CTCs from mCRC patients. PMID: 26950035 Taxus chinensis var.-mediated downregulation of MRP1, MDR1, and LRP might contribute to the reversal of drug resistance in non-small cell lung cancer stem cells. PMID: 27706681 miR-145 accelerated MRP1 mRNA degradation by directly targeting its 3'-UTR and therefore caused increased cisplatin toxicity in gallbladder cancer cells. Lower miR-145 and higher MRP1 expression levels predicted poor prognosis in GBC patients who received chemotherapy. PMID: 26852750 Caveolin-1 affects ESCC MDR by regulating the expressions of P-gp and MRP1. PMID: 26768616 Report ABCC1 induction by genistein in breast cancer cells may result in drug resistance in tumors treated with substrates of this transport. PMID: 27033456 Results suggest that the LRIG1could negatively control MRP-1 and the apoptosis to improve the sensitivity of VP16-related chemotherapy. PMID: 27183435 Data, including data from studies in knockout mice, suggest that MRP1 is involved in development of vascular endothelial dysfunction in diabetic angiopathy; MRP1 appears to be required for oxidative stress in endothelial cells due to hyperglycemia. PMID: 26908299 The expression of ABCB1, ABCC1 and ABCG2 on primary human T-cells correlates with the efficacy of JAK inhibitor ruxolitinib treatment in regard to T-cell activation and proliferation. PMID: 26589910 Positive expressions of MRP and TOP2A in the tumor tissue are associated with increased risk of developing brain metastases in non-small cell lung cancer (NSCLC). PMID: 26617887 the expression and localization of the ATP-Binding Cassette (ABC) transporters BCRP (ABCG2), P-gp (ABCB1) and MRP1 (ABCC1) in human umbilical cords, is reported. PMID: 26407213 Report decreased function MRP1 activity in methotrexate treated rheumatoid arthritis patients. PMID: 26715450 the specific COX-2 inhibitor meloxicam can increase the intracellular accumulation of doxorubicin and enhance doxorubicin-induced cytotoxicity in A549 cancer cells by reducing the expression of MRP1 and MRP4. PMID: 26600514 The scanning study identified 46 polymorphic variants by screening 30 exons of the ABCC1 gene with adjacent intronic fragments in 190 unrelated Polish individuals. A large fraction of variants represented rare or even completely novel ones. PMID: 26395522 ILK may have an important role in the progression of NSCLC, possibly through up-regulation of Snail and MRP1. PMID: 25964055 MRP1 knockdown down-regulates the deposition of collagen and leads to a reduced hypertrophic scar fibrosis PMID: 26092470 MRP1 activity showed no significant change I primary colorectal cancer patients. PMID: 25885226 Chronic arsenic exposure negatively correlates with the expression of MRP1 in bronchoalveolar lavage cells in patients with lung cancer. PMID: 26031227 The present study investigated the time course and dose dependency of the induction of three efflux proteins, P-gp, MRP1 and MRP5, in response to gemcitabine exposure in Capan-2 pancreatic cancer cell line at transcriptional and translational levels. PMID: 25564970 Our data suggested that miR-7 mediated small cell lung cancer chemoresistance by repressing MRP1/ABCC1 PMID: 26108539 study shows that genetic variability in the nucleotide binding domain 1 has a significant impact on the ABCC1 transcript level in the target tissue and may modify survival of breast cancer patients PMID: 25078270 The level of FBW7 expression in CNE2 cells was correlated with CDDP chemosensitivity. siRNA-mediated upregulation of FBW7 expression downregulated the expression of MRP, significantly increasing drug sensitivity in CNE2 cells. PMID: 25586348

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.