Recombinant Human MSRB3 Protein (His Tag)

Name: Recombinant Human MSRB3 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-3411
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	Q8IXL7
Synonym	DFNB74
Background	The affected individuals of six apparently unrelated families were homozygous for the same transversion (c.265T>G) in MSRB3, which encodes a zinc-containing methionine sulfoxide reductase B3. This cysteine residue of MSRB3 is conserved in orthologs from yeast to humans and is involved in binding structural zinc. Mouse Msrb3 is expressed widely. In the inner ear, it is found in the sensory epithelium of the organ of Corti and vestibular end organs as well as in cells of the spiral ganglion. MSRB3-catalyzed reduction of methionine sulfoxides to methionine is essential for hearing.
Description	A DNA sequence encoding the human MSRB3(Q8IXL7-2) (Gly26-Leu185) was expressed with a His tag at the N-terminus.
Source	E.coli
Predicted N Terminal	His
AA Sequence	Gly26-Leu185
Molecular Weight	The recombinant human MSRB3 consists of 178 a.a. and predicts a molecular mass of 19.6 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine. Isoform 2 is essential for hearing.
Subcellular Location	[Isoform 1]: Endoplasmic reticulum.; [Isoform 2]: Mitochondrion.
Protein Families	MsrB Met sulfoxide reductase family
Database References	HGNC: 27375 OMIM: 613718 KEGG: hsa:253827 STRING: 9606.ENSP00000347324 UniGene: Hs.339024
Associated Diseases	Deafness, autosomal recessive, 74 (DFNB74)
Tissue Specificity	Widely expressed.

Gene Functions References

we characterise the mammalian enzyme Msr B3. There are two splice variants of this enzyme that differ only in their N-terminal signal sequence, which directs the protein to either the endoplasmic reticulum (ER) or mitochondria PMID: 29420254
This characterization of GWAS-implicated MSRB3 protein expression in human hippocampus suggests that patterns of neuronal and vascular MsrB3 protein expression reflect or underlie pathology associated with Alzheimer disease. PMID: 28777754
Oncogene induction in differentiated cells induces massive DNA damage, mammary stem cells are resistant, owing to a preemptive program driven by ZEB1 and MSRB3. The prevention of oncogene-induced DNA damage precludes induction of the oncosuppressive p53-dependent DNA-damage response, thereby increasing stem cells' intrinsic susceptibility to malignant transformation. PMID: 28394329
The data suggest that MsrB3 attenuates HO-1 induction by inhibiting reactive oxygen species production, endoplasmic reticulum stress, and Nrf2 activation. PMID: 27059143
MsrB3 plays an important role in cancer cell survival through the modulation of the intrinsic apoptosis pathway. PMID: 28007593
Taken together, our results suggest that MsrB3 plays a critical role in cancer cell apoptosis through the modulation of ER stress status. PMID: 28389299
MsrB3 deficiency activates the cell cycle inhibitors p21 and p27. PMID: 24583268
DNA methylation shows genome-wide association of NFIX, RAPGEF2 and MSRB3 with gestational age at birth. PMID: 22422452
these data provide evidence that the ER-type of MsrB3 plays an important role in protection against ER stress, suggesting that MsrB3 may be involved in the regulation of ER homeostasis. PMID: 22405767
Taken together, these data provide evidence that the ER type of MsrB, MsrB3A, plays an important role in protection mechanisms against oxidative, cold and heat stresses and, moreover, in the regulation of fruit fly aging. PMID: 22310715
Results identified an antimicrobial peptide from the human methionine sulfoxide reductase B3 protein. PMID: 22027001
an in vitro assay revealed that p.Cys89Gly completely abolished MSRB3 enzymatic activity and that p.Arg19X is a null allele for MSRB3 mitochondrial isoforms, indicating that DFNB74 deafness might be a mitochondrial disease limited to the inner ear. PMID: 21185009
Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 20195514

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.