Recombinant Human MD1 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-3317

Recombinant Human MD1 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-3317
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Product Overview

Tag Fc
Host Species Human
Accession O95711
Synonym dJ80N2.1, MD-1, MD1, MMD-1
Background MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 15 (RP15) which is a molecule containing leucine-rich repeats and is expressed on B cells, dentritic cells and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP15 cell surface expression and function. It is indicated that the RP15/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 4% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney.
Description A DNA sequence encoding the human LY86 (O95711) (Met1-Ser162) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Gly 21
AA Sequence Met1-Ser162
Molecular Weight The recombinant human LY86/Fc is a disulfide-linked homodimer. The reduced monomer comprises 383 a.a. and has a predicted molecular mass of 42.7 kDa. The apparent molecular mass of the protein is approximately 48 kDa in SDS-PAGE under reducing conditions
Purity >85% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression.
Subcellular Location Secreted, extracellular space. Note=Associated with CD180 at the cell surface.
Database References
Tissue Specificity Highly expressed in B-cells, monocytes and tonsil.

Gene Functions References

  1. our results reveal that MD-1 deficiency is of critical importance in down-regulating induction and progression of colitis, thereby suggesting that MD-1 might be a target for future interventional therapies of inflammatory bowel disease. PMID: 27209086
  2. DNA methylation of the LY86 gene is associated with obesity, insulin resistance, and inflammation. PMID: 24735745
  3. MD-1 may be a disease susceptibility gene for adult asthma in a Southern Han population in China. PMID: 21426727
  4. Both mouse and human RP105/MD-1 exhibit dimerization of the 1:1 RP105/MD-1 complex, demonstrating a novel organization. PMID: 21959264
  5. In this study, we produced variants of MD-1 and MD-2 in Pichia pastoris. Contrary to previous reports, this study suggests that MD-1 can bind to LPS. PMID: 21130168
  6. in the promoter region we identified 3 SNPs, rs1334710, rs4959389 and rs977785 that are associated with mite-sensitive allergy in Taiwanese children; results suggested that MD-1 could be a susceptible gene for mite-sensitive allergy in Taiwanese children PMID: 18001295
  7. Identified MD-1 SNP (rs7740529) association with asthma in Taiwanese children and adults. PMID: 19255686


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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