Recombinant Human MAP4K5 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3286
Recombinant Human MAP4K5 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3286
Collections: Other recombinant proteins, Recombinant proteins
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Product Overview
Tag | His&GST |
Host Species | Human |
Accession | NP_006566.2 |
Synonym | GCKR, KHS, KHS1, MAPKKKK5 |
Background | Mitogen-activated protein kinase kinase kinase kinase 5, also known as Kinase homologous to SPS1/STE2, MAPK/ERK kinase kinase kinase 5, MEK kinase kinase 5 and MAP4K5, is a cytoplasm protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE2 subfamily. MAP4K5 is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate. It contains oneCNH domain and oneprotein kinase domain. MAP4K5 is highly similar to yeast SPS1/STE2 kinase. Yeast SPS1/STE2 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. MAP4K5 has been shown to interact with CRKL and TRAF2. This kinase was shown to activate Jun kinase in mammalian cells. MAP4K5 is an early component of MAP kinase signal cascades. It may play a role in the response to environmental stress. MAP4K5 appears to act upstream of the JUN N-terminal pathway. |
Description | A DNA sequence encoding the human MAP4K5 (NP_006566.2) (Met 1-Tyr 846) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | Met |
AA Sequence | Met 1-Tyr 846 |
Molecular Weight | The recombinant human MAP4K5/GST chimera consists of 1083 a.a. and has a calculated molecular mass of 123 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions. |
Purity | >82% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation | Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM TCEP, pH 8.5. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |