Recombinant Human LR3-IGF-1 Protein

Name: Recombinant Human LR3-IGF-1 Protein
Brand: Beta LifeScience
SKU: BL-1689NP
Availability: InStock

BL-1689NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

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Product Overview

Description	Recombinant Human LR3 Insulin-Like Growth Factor-I is produced by our E.coli expression system and the target gene encoding Gly49-Ala118 is expressed.
Accession	P05019
Synonym	Insulin-Like Growth Factor I; IGF-I; Mechano Growth Factor; MGF; Somatomedin-C; IGF1; IBP1
Gene Background	Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contains the N- and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids has 94% identity with mouse IGF-I and exhibits cross-species activity. IGF-1 binds IGF-IR, IGF-IIR, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone. R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. R3 IGF-1 has been produced with the purpose of increasing biological activity. R3 IGF-1 is significantly more potent than human IGF-I in vitro.
Molecular Mass	9.1 KDa
Apmol Mass	11 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, 4% Mannitol, pH 4.5.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in 50mM Acetic Acid.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
Subcellular Location	Secreted.
Protein Families	Insulin family
Database References	HGNC: 5464 OMIM: 147440 KEGG: hsa:3479 STRING: 9606.ENSP00000302665 UniGene: PMID: 29530981 We found that elevated production of insulin-like growth factor 1 by polyploid ASCs rendered them more potent in tumor growth promotion in vitro. PMID: 30185144 No up-regulation of Wnt10A and IGF-1 mRNA was observed with 1,550-nm Er:Glass fractional laser treatment of androgenetic alopecia. PMID: 30096107 Significant negative or positive correlations were observed between IGF-1 concentrations and impairments on several EDI-2 subscales (drive for thinness, body dissatisfaction, interoceptive awareness, sense of ineffectiveness, interpersonal distrust, maturity fear) and on SCL-90 subitems (depression, hostility, obsessivity compulsivity, anxiety), suggesting a possible hormonal modulatory effect on specific aspects of eatin PMID: 29179911 This article reviews the tumor-specific molecular signatures of IGF-1-mediated epithelial-mesenchymal transition in breast, lung, and gastric cancers. [review] PMID: 30111747 Results provide evidence for the role of IGF-I: matrix protein interactions in cell growth, migration and melanoma progression through forming a complex with IGF binding proteins. PMID: 29330502 The molecular interactions of IGF1-IGF1R binding have been dissected. PMID: 29483580 genetic association and nutrigenomic studies in population of postmenopausal women in China: Data confirm that dietary acid load is associated with postmenopausal osteoporosis; an SNP in IGF1 (rs35767) is not associated with this relationship in the population studied. PMID: 30018240 Low IGF-I levels are associated with non-alcoholic fatty liver disease. PMID: 29395967 Low IGF1 expression is associated with retinopathy of prematurity. PMID: 29274846 Low IGF-I levels are associated with dry eye syndrome. PMID: 29452886 serum IGF-1 is associated with more muscle mass, higher bone mineral density, and better handgrip performance in both genders among community-dwelling middle aged and older adults in Taiwan PMID: 28351218 insulin-like growth factor 1 (IGF1) is a direct target of miR-1827. PMID: 28387248 Results found that the expression of IGF-1 was up-regulated in cumulus cells (CCs) of women with polycystic ovary syndrome (PCOS), which was inversely proportional to the expression level of miR-323. Also, IGF1 3'UTR is targeted by miR-323. PMID: 30300681 Study in hepatocellular carcinoma cell line elicited a new mechanism in which IGF-1 induced epithelial-mesenchymal transition through regulation of survivin and a downstream pathway. PMID: 29989646 in this study, the influence of IGF-1 and BMP-7 in different concentrations on the osteogenic differentiation of two human MSC-subtypes, isolated from reaming debris (RMSC) and iliac crest bone marrow (BMSC) has been assessed PMID: 29874864 AMH, IGF1 and leptin levels in follicular fluid have no relation to the fertility disorders caused by endometriosis or fallopian tube damage, though they are biomarkers for anovulatory fertility disorders. PMID: 29595066 pituitary growth hormone secretory capacity is not related to the IGF-1 levels PMID: 29098662 Peripheral totalIGF-1 and IGFBP-3 were associated with better performance in attention, visuospatial, and global cognitive domains, independent of the gait speed. PMID: 29547749 CA repeat polymorphism of the P1 promoter of the IGF1 gene was associated with strength predispositions in the homozygous and non-carriers groups. In the group who were heterozygous it was speed-strength aptitudes. PMID: 29543920 IGF1 levels in systemic lupus erythematosus patients decline with age at a similar rate as in healthy controls, are associated with positive metabolic effects and despite a moderating effect on B, NK and CD8+ cells, do not affect clinical disease activity or severity. PMID: 29385899 results indicate that IGF-1 gene polymorphisms play crucial roles in the histopathological progression of IgA Nephropathy in the Chinese Han population PMID: 29402846 Low IGF1 level is associated with steatosis in Pituitary Diseases. PMID: 29065431 They retained the main IGF-1R-related properties, but the hormones with His49 in IGF-1 and His48 in IGF-2 showed significantly higher affinities for IR-A and for IR-B, being the strongest IGF-1- and IGF-2-like binders of these receptors ever reported. PMID: 29608283 Mechanism of activation of SGK3 by IGF1 via the class 1 and class 3 phosphatidylinositol 3-kinases has been described. PMID: 29150437 IGF1 is associated with giant cell tumor of bone recurrence, which might serve as biomarker for giant cell tumor of bone recurrence PMID: 29651441 miR-19b is decreased in PCOS granulosa cells and miR-19b could be a granulosa cell proliferation inhibitor. miR-19b-mediated cell proliferation may act via directly targeting IGF-1. PMID: 29363717 Report deregulation of IGF1/IGFBP3 expression in breast cancer correlating with neoplasm staging and histologic grade. PMID: 30084561 Study observed that the IGF1 level was higher in human epithelial ovarian cancer (EOC) specimens than that in benign ovarian tumor specimens, and further analysis showed that a higher level of IGF1 was related to more advanced clinical stage and liver metastasis. Also, up-regulation of IGF1 by tumor-associated macrophages was shown to promote the proliferation and migration of EOC cell lines. PMID: 29251331 Overexpression of circHIPK3 increased the expression levels of IGF1 and knock-down reduced it. PMID: 28738961 0001) only. Women who remained obese had increased cGP/IGF-1 ratio (p = 0.006) only. Increase in cGP/IGF-1 ratio is associated with obesity, but not hypertension. Changes of IGFBP-3 and/or cGP/IGF-1 ratio are associated with weight changes. The data suggest the role for cGP in obesity through autocrine regulation of IGF-1. PMID: 29921371 IGF-1 exerts antioxidant, anti-inflammatory and protective effects on the central nervous system. (Review) PMID: 29975480 Five single nucleotide polymorphisms (SNPs) of IGF-1 (rs6214, rs6218, rs35767, rs5742612, and rs5742714) were genotyped. DNA was extracted from peripheral blood and analyzed for SNP genotyping using PCR. rs6218 had a predictive role for the susceptibility and progression of osteosarcoma. The TC and CC genotypes of rs6218 indicated higher risk of osteosarcoma; associated with later stage and elevated risk of osteosarcoma PMID: 29232358 Bio informatics analysis of 982 lung cancer patients revealed that higher expression of TNF- alpha was associated with low risk of cancer progression while overexpression of IGF-1 was correlated with high risk. Collectively, these results reveal that the cytokines in the tumor microenvironment differentially modulate radiation therapy through a variety of signaling mechanisms. PMID: 27344406 Data suggest that lower IGF1 serum levels in aging are associated with lower handgrip strength and worse physical performance, but less recurrent falls, especially in men. This longitudinal study was conducted in the Netherlands. PMID: 29789408 Data suggest that R1353H point mutation constitutes an activating IGF1R variant that can result in extreme tall height with very low levels of serum IGFI; the mutation was observed in proband, his mother, and his 3 sons, however, only the proband (43 y/o) and eldest son (18 y/o) exhibit extreme tall height. [CASE REPORT] PMID: 29789409 IGF-1 treatment activated protein kinase B (AKT), which may inhibit autophagy via the AKT/mammalian target of rapamycin signaling pathway. Following inhibition of autophagy, drug resistant cells became sensitive to apoptosis induced by 5-fluorouracil. PMID: 29257307 Consistent with its enhanced expression in Laron syndrome, we provide evidence that TXNIP gene expression is negatively regulated by IGF1. PMID: 29339473 In elderly women with low-energy distal radius fractures, an association between IGF1 and lowest measures of bone mineral density was found, indicating that low IGF1 could be an indirect risk factor for fractures. PMID: 29178313 Expression of miR-30a-3p was significantly increased in the placentas of patients with preeclampsia. miR-30a-3p might be involved in the pathogenesis of preeclampsia by targeting IGF-1 and regulating the invasion and apoptosis of trophoblast cells. PMID: 29155142 results suggest that the different isoforms of the PI3K p110 subunit could be therapeutic targets for primary and metastatic colon cancer and that regulation of the NRD1/ADAM signaling pathway controls lipogenesis-mediated EMT in IGF-1-stimulated colon cancer cells. PMID: 28819788 Demonstrate the uptake of fluorescently labeled IGF-I into skeletal growth plates of live mice using multiphoton microscopy. PMID: 28798204 Study provides evidence that IGF-1/IGF-1R/hsa-let-7c axis can control the odonto/osteogenic differentiation of IGF-1-treated stem cells from apical papilla via the regulation of JNK and p38 MAPK signaling pathways. PMID: 27833148 Circulating sex steroids, prolactin, insulin-like growth factor (IGF) I, IGF-binding protein 3, and sex hormone-binding globulin (SHBG) were evaluated using backward elimination separately in women pre- and postmenopausal at blood collection. PMID: 28246273 association between serum IGFBP-1 and IGF-I levels with advanced fibrosis in non-alcoholic fatty liver disease patients PMID: 28927302 findings suggest that the IGF1 polymorphism rs5742714 may be a genetic predictor of susceptibility and prognosis of renal cell carcinoma. PMID: 27976731 The results of the study may help to inform future health interventions that utilize physical activity as a means to improve cognitive development in children, adolescents, and adults. Additionally, the study may assist in determining whether the putative effects occur via modification of plasma IGF-1 or BDNF concentrations. PMID: 29094050 Data show the differential expression of insulin like growth factor 1 (IGF-I) transcripts isoforms in bladder cancer, revealing a distinct suppression of IGF-IEc. PMID: 29848696 Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes. PMID: 28467017 Human IGF-I propeptides and mutants were overexpressed in bovine articular chondrocytes. Secreted IGF-I propeptides stimulated articular chondrocyte biosynthetic activity as much as mature IGF-I. Of the 3 IGF-I propeptides, only proIGF-IA strongly bound to heparin, depending on N-glycosylation at Asn92 in the EA peptide. PMID: 29174671

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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