Recombinant Human LOXL4 Protein

Beta LifeScience SKU/CAT #: BL-1166SG

Recombinant Human LOXL4 Protein

Beta LifeScience SKU/CAT #: BL-1166SG
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Product Overview

Tag GST
Host Species Human
Accession BC013153
Synonym LOXC, FLJ21889
Background LOXL4 or lysyl oxidase-like 4 is a member of the lysyl oxidase family of amine oxidases. LOXL4 catalyzes the final enzymatic step required for the lysine-derived crosslinks essential for the formation of collagen fibrils and insoluble elastic fibers within the extracellular matrix (1). LOXL4 can also contribute to developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis. LOXL4 is highly expressed in skeletal muscle, testis, and pancreas (2).
Description Recombinant human LOXL4 (492-end)- was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source Sf9 insect cells
AA Sequence 492a.a.-end
Molecular Weight 56 kDa
Purity For specific purity information on a given lot, see related COA.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability The recombinant protein is stable for up to 12 months at -70°C
Usage For Research Use Only
Storage Recombinant Human LOXL4 Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function May modulate the formation of a collagenous extracellular matrix.
Subcellular Location Secreted, extracellular space.
Protein Families Lysyl oxidase family
Database References

HGNC: 17171

OMIM: 607318

KEGG: hsa:84171

STRING: 9606.ENSP00000260702

UniGene: PMID: 27597564

  • Although expression of LOXL4 is not statistically associated with neck metastases, we showed that LOXL4 expression significantly increased in laryngeal cancer. PMID: 26138381
  • In hepatocellular carcinoma, low LOXL4 expression was associated with lower overall survival rates and higher cumulative recurrence rates. PMID: 26097573
  • These results provide a set of the essential genes in this newly identified miR-193a-3p/LOXL4/Oxidative Stress axis. PMID: 25311867
  • High Lysyl oxidase-like 4 promotes proliferation and metastasis of gastric cancer via FAK/Src pathway. PMID: 25216702
  • data suggest that abnormal expression of LOXL4 of KCOT may enhance angiogenesis in KCOT, which may help to promote the locally aggressive biological behavior of KCOT. PMID: 24357854
  • TGF-beta1 induced injured MCL to express more LOXs than injured ACL (up to 1.85-fold in LOX, 2.21-fold in LOXL-1, 1.71-fold in LOXL-2, 2.52-fold in LOXL-3 and 3.32-fold in LOXL-4). PMID: 23357697
  • Alternatively spliced lysyl oxidase-like 4 isoforms have a pro-metastatic role in cancer. PMID: 22806361
  • The human lysyl oxidase-like 4 gene does not confer increased genotypic risk for adolescent idiopathic scoliosis. PMID: 21740577
  • SNPs in the lysyl oxidase-like protein 4 and complement component 3 genes are associated with increased risk for endometriosis and endometriosis-associated infertility in a Puerto Rican population. PMID: 21733505
  • Overexpression of the lysyl oxidase-like 4 gene is associated with metastatic head/neck carcinoma. PMID: 21115907
  • The data presented in this work might emphasize the involvement of LOXL4 in molecular processes of the genesis or progression of head and neck carcinomas. PMID: 12894545
  • LOXL4, with the four scavenger receptor cysteine rich domains, may also function as an active amine oxidase PMID: 14551188
  • Up-regulation of lysyl oxidase mRNA and protein expression is associated with oral squamous cell carcinoma PMID: 17671119
  • LOXL4-DC stimulated T cells produce higher IFN-gamma secretion compared to unstimulated T cells and T cells stimulated with untransfected DCs, in the presence of the pan-DR-epitope (PADRE) PMID: 18202753
  • These results suggest that LOXL4 may function as a negative feedback regulator of TGF-beta1 in cell invasion by inhibiting the metabolism of extracellular matrix (ECM) components. PMID: 18586005
  • Functional analysis of the 5' flanking domain of the LOXL4 gene in head and neck squamous cell carcinoma cells. PMID: 18949373
  • In breast carcinoma, LOXL4 was expressed only in the effusion samples. In malignant mesothelioma, LOXL4 and its splice variants were expressed at all sites. PMID: 19015874
  • FAQs

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    Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

    Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

    Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

    Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

    To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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