Recombinant Human LOK Protein
Beta LifeScience
SKU/CAT #: BL-0145SG
Recombinant Human LOK Protein
Beta LifeScience
SKU/CAT #: BL-0145SG
Catalog No.: BL-0145SG
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | NM_005990 |
| Synonym | STK10, PRO2729 |
| Background | LOK is a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. LOK can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression (1). LOK can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. MEKK1 and LOK have opposing roles in regulating the enhancer element of the IL-2 gene during T-cell activation. While MEKK1 is responsible for the CD28 signalling pathway that activates the CD28 response element, co-expression of LOK attenuates these effects (2). |
| Description | Recombinant full-length human LOK was produced in Sf9 cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. |
| Source | Sf9 insect cells |
| AA Sequence | Full Length |
| Molecular Weight | ~138 kDa |
| Purity | For specific purity information on a given lot, see related COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Active |
| Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
| Stability | The recombinant protein is stable for up to 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Recombinant Human LOK Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
