Recombinant Human LIN28B Protein

Beta LifeScience SKU/CAT #: BL-3930PS
Recombinant Human LIN28B Protein

Recombinant Human LIN28B Protein

Beta LifeScience SKU/CAT #: BL-3930PS
Catalog No.: BL-3930PS

Product Overview

Tag N/A
Host Species Human
Synonym Lin-28 Homolog B (C. Elegans), Protein Lin-28 Homolog B, CSDD2, Lin-28B, Lin-28.2, FLJ16517.
Background Lin28B is a member of the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 mRNA. Lin28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. LIN28 is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines.
Description LIN28B Human Recombinant expressed in E. coli is a single polypeptide chain containing 273a.a. (1-250) and having a molecular weight of 29.5 kDa. LIN28B is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT.
Purity >85% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The LIN28B solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 2mM DTT and 10% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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