Recombinant Human L-Xylulose Reductase (DCXR) Protein (GST)

Name: Recombinant Human L-Xylulose Reductase (DCXR) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-08807P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human L-Xylulose Reductase (DCXR) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q7Z4W1
Target Symbol	DCXR
Synonyms	Carbonyl reductase 2; Carbonyl reductase II; DCR; Dcxr; DCXR_HUMAN; Dicarbonyl and L xylulose reductase; Dicarbonyl L xylulose reductase; Dicarbonyl/ L xylulose reductase; Dicarbonyl/L-xylulose reductase; HCR2; HCRII; Human carbonyl reductase 2; kiDCR; Kidney dicarbonyl reductase; L xylulose reductase; L-xylulose reductase; P34H; PNTSU; SDR20C1; Short chain dehydrogenase/reductase family 20C member 1; Sperm surface protein P34H; XR
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	MELFLAGRRVLVTGAGKGIGRGTVQALHATGARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERALGSVGPVDLLVNNAAVALLQPFLEVTKEAFDRSFEVNLRAVIQVSQIVARGLIARGVPGAIVNVSSQCSQRAVTNHSVYCSTKGALDMLTKVMALELGPHKIRVNAVNPTVVMTSMGQATWSDPHKAKTMLNRIPLGKFAEVEHVVNAILFLLSDRSGMTTGSTLPVEGGFWAC
Expression Range	1-244aa
Protein Length	Full Length
Mol. Weight	52.9kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
Subcellular Location	Membrane; Peripheral membrane protein.
Protein Families	Short-chain dehydrogenases/reductases (SDR) family
Database References	HGNC: 18985 OMIM: 260800 KEGG: hsa:51181 STRING: 9606.ENSP00000303356 UniGene: PMID: 28012250 identification of DCXR as an enzyme activity mediating chemical redox cycling suggests that it may be important in generating cytotoxic reactive oxygen species in the lung PMID: 28595002 Low expression of DCXR is associated with hepatocellular carcinoma. PMID: 27658779 EGCG is a potential inhibitor to human DCXR. PMID: 23661708 combined frequency of the two mutant DCXR alleles in 1,067 Ashkenazi Jewish controls was 0.0173, suggesting a pentosuria frequency of approximately one in 3,300 in this population PMID: 22042873 highly expressed in kidney and liver PMID: 11882650 crystallization and preliminary crystallographic analysis PMID: 12136162 structure of the tetrameric form of human L-Xylulose reductase PMID: 15906319 Our results demonstrate that the proportion of positive P34H cases that produced embryos in vitro clearly differs from cases with undetectable levels of P34H (P<.001). PMID: 16616743 These results suggested that altered HCR2 expression might play roles in the carcinogenesis and progression of hepatocellular carcinoma. PMID: 16847567 A significant proportion of men investigated for male infertility may be epididymal protein P34H deficient. PMID: 17434498 Decreased membranous expression of DCXR with altered subcellular localization appears to be associated with malignant progression of melanocytic lesions in human skin. PMID: 17576332 DCXR trranfected into mice may function in the removal of renal alpha-dicarbonyl compounds under oxidative circumstances, but it is not sufficient to suppress acute renal fibrosis. PMID: 18079483 DCXR overexpression has the potential to be an additional useful biomarker for prostate cancer. PMID: 18086765 The action of human XR may be regulated by cellular redox conditions through reversible disulfide-bond formation and by S-cysteinylation. PMID: 19337691

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.