Recombinant Human IRAK4 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3016
Recombinant Human IRAK4 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3016
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | Q9NWZ3 |
Synonym | IPD1, IRAK-4, NY-REN-64, REN64 |
Background | Interleukin-1 receptor-associated kinase 4, also known as Renal carcinoma antigen NY-REN-64, IRAK-4 and IRAK4, is a member of the protein kinase superfamily, TKL Ser/Thr protein kinase family and Pelle subfamily. IRAK4 contains onedeath domain and oneprotein kinase domain. IRAK4 is required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. It also phosphorylates IRAK1. A member of the IL-1 receptor (IL-1R)-associated kinase (IRAK) family, IRAK4, has been shown to play an essential role in Toll-like receptor (TLR)-mediated signaling. IL-1-mediated IRAK4 kinase activity in T cells is essential for induction of IL-23R expression, Th17 differentiation, and autoimmune disease. Pharmacological blocking of IRAK4 kinase activity will retain some levels of host defence, while reducing the levels and duration of inflammatory responses, which should provide beneficial therapies for sepsis and chronic inflammatory diseases. Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) which is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD. Defects in IRAK4 are also the cause of IRAK4 deficiency which causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children. |
Description | A DNA sequence encoding the full length of human IRAK4 (Q9NWZ3-1) (Met 1-Ser 460) was expressed with a His tag at the N-terminus. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | Met |
AA Sequence | Met 1-Ser 460 |
Molecular Weight | The recombinant human IRAK4 consists of 478 a.a. and predicts a molecular mass of 53.8 kDa. It migrates as an approximately 48 kDa band in SDS-PAGE under reducing conditions. |
Purity | >92% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | The specific activity was determined to be 169 nmol/min/mg using MBP as substrate. |
Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |