Recombinant Human Interleukin-20 Protein (IL20), Active

Beta LifeScience SKU/CAT #: BLC-05389P

Recombinant Human Interleukin-20 Protein (IL20), Active

Beta LifeScience SKU/CAT #: BLC-05389P
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Product Overview

Description Recombinant Human Interleukin-20 Protein (IL20), Active is produced by our E.coli expression system. This is a full length protein.
Purity Greater than 95% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x10 6 IU/mg.
Uniprotkb Q9NYY1
Target Symbol IL20
Synonyms Cytokine Zcyto10; Four alpha helix cytokine; IL-20; IL10D; IL20; IL20_HUMAN; Interleukin 20; Interleukin-20; MGC96907; UNQ852/PRO1801; ZCYTO10
Species Homo sapiens (Human)
Expression System E.coli
Tag Tag-Free
Complete Sequence M+LKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE
Expression Range 25-176aa
Protein Length Full Length of Mature Protein
Mol. Weight 17.6 kDa
Research Area Immunology
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.2, with trehalose
Reconstitution Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3.
Subcellular Location Secreted.
Protein Families IL-10 family
Database References

HGNC: 6002

OMIM: 605619

KEGG: hsa:50604

STRING: 9606.ENSP00000356063

UniGene: PMID: 28514748

  • The authors results indicate that genetic variants of the immunomodulatory IL10 and IL20 genes may offer a protective effect in Europeans from Russia. PMID: 28150860
  • data showed that IL-20 is independently associated with rheumatoid arthritis disease activity and may be triggered by TLR ligands at local sites of inflammation PMID: 28662439
  • IL-20 is a multifunctional cytokine with potent inflammatory, angiogenic, chemoattractive, and osteoclastogenic effects, and is involved in the many phases of rheumatic arthritis progression. [Review] PMID: 26297177
  • Data suggest that HGF, IL-20, and IL-22 in the serum and bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) patients before chemotherapy may be a prognostic of cancer progression. PMID: 27573644
  • The current study provides evidence that IL-20 directly targets and inhibits key inflammatory functions of neutrophils during infection with S. aureus. PMID: 28424238
  • Results found that IL-20 is more specifically associated with ER-positive breast cancer and is epigenetically regulated by estrogen via the specific recruitment of KMT2B by ERalpha. PMID: 27806114
  • Coincident diabetes mellitus type 2 in either pulmonary or latent tuberculosis is characterized by decreased production of the IL-20 subfamily of cytokines. PMID: 26354610
  • IL-20 serum levels were increased in patients with active multiple myeloma compared to both healthy controls and responders to bortezomib-based treatment.IL-20 participate actively in the pathophysiology of multiple myeloma progression. PMID: 25631632
  • Overexpression of IL-20 was detected in the airway epithelium collected from patients with asthma. PMID: 25028099
  • IL-20 may play a patho-physiologic role in the Th2 immune response in human asthma and may be a potential biomarker of asthma severity. PMID: 25543042
  • IL-20 is produced by keratinocytes, released into the epidermis and then possibly taken up by papillary mononuclear cells PMID: 24628979
  • Decreased interleukin-20 expression in scleroderma skin contributes to cutaneous fibrosis. PMID: 24470401
  • We observed that IL-20 which is an IL-10 group angiogenesis indicator was suppressed in systemic sclerosis, suggesting abnormal angiogenesis. PMID: 23812620
  • This study demonstrates that rs1713239 and infection may have potential synergetic effect on modulating the transcriptional activity of IL-20. PMID: 23892591
  • The mRNA levels of p40, IL-20 and IL-21 at baseline may serve as potential predictors of treatment response to ustekinumab treatment in psoriasis. PMID: 22930279
  • IL-10-- and IL-20--expressing epithelial and inflammatory cells are increased in patients with ulcerative colitis. PMID: 23207823
  • the GG genotypes of the IL-20 polymorphisms (rs2981573 and rs2232360) might have an important role in the development of UC in the Mexican population. PMID: 23183096
  • A blood test for discrimination of patients with acute cellular rejection from transplanted kidney is based on simultaneous quantification of three analytes: IL-1 receptor antagonist, IL-20 and soluble CD40 ligand. PMID: 22948742
  • Interleukin-20 promotes migration of bladder cancer cells through extracellular signal-regulated kinase (ERK)-mediated MMP-9 protein expression leading to nuclear factor (NF-kappaB) activation by inducing the up-regulation of p21(WAF1) protein expression PMID: 23271730
  • Elevated expression of inflammatory cytokines (IL-5, IL-20, and IL-28A) is associated with bladder cancer development. PMID: 22962576
  • crystallographic asymmetric unit contains one IL-20-IL-20R1-IL-20R2 complex, corresponding to a solvent content of approximately 54% PMID: 22232181
  • IL-20 & its receptors were epigenetically regulated through histone post-translational modifications and DNA CpG residue methylation. Also, treatment with recombinant IL-20 resulted in decreased expression of the VEGF family members at the mRNA level. PMID: 21565488
  • The G allele of the IL-20 -1723C to G polymorphism may contribute to a genetic susceptibility to psoriasis in the Chinese Han population, particularly in patients with psoriasis triggered or exacerbated by an upper respiratory tract infection. PMID: 21109726
  • in premenopausal obese women, interleukin-20 levels are higher than matched normal weight control women, are associated with body weight and waist-hip ratio, and are reduced by weight loss PMID: 19481430
  • Essentially identical phenotypes in transgenic mouse models for IL-20, IL-22, and IL-24 suggest that the three related cytokines play a redundant role in keratinocyte differentiation and proliferation. PMID: 20061404
  • human IL-20 enhanced colony formation by CD34+ multipotential progenitors, buthad no effect on erythroid, granulocyte-macrophage, or megakaryocyte progenitors. PMID: 12855566
  • IL-19 and IL-20 are synthesized by a distinct population of keratinocytes. PMID: 14675174
  • Altogether our findings revealed that IL-20 is a negative modulator of COX-2/PGE(2) and inhibits angiogenesis. PMID: 15950941
  • IL-20 is a pleiotropic cytokine and promotes angiogenesis. PMID: 16511554
  • IL-20 may influence inflammation through IFN-like effects. These data indicate that IL-20 may be an important effector cytokine in psoriasis, and that its inhibition may represent a potential therapeutic target. PMID: 16645593
  • Ultraviolet light with photosensitizing agents had little effect or decreased epithelial keratinocyte il-20 levels. PMID: 16709143
  • IL-20 is a proatherogenic cytokine that contributes to the progression of atherosclerosis. PMID: 16778121
  • IL-20 protein is expressed in 30 non-neoplastic tissue types and five major cell types: epithelial cells, myoepithelial cells, endothelial cells, macrophages, skeletal muscle cells, and several types of tumor cells. PMID: 16908179
  • p38 mitogen-activated protein kinase (MAPK) as well as inhibitory kappaB kinase-NF-kappaB signaling pathways are crucial for IL-20 expression in keratinocytes PMID: 17255956
  • Review. The role of IL-20 in the pathogenesis of inflammatory diseases is reviewed. PMID: 17465720
  • Activation of toll-like receptors 2 and 3 show keratinocytes in the simultaneous presence of IL-20 and IL-29. PMID: 18281438
  • results suggest that IL10 and IL20 gene variants influence hepatitis B virus infection outcome PMID: 18479293
  • IL-20 plays an important role in the pathogenesis of disc herniation. PMID: 18758357
  • novel effects of IL-20 on mesangial cells and its association with lupus nephritis. PMID: 18771958
  • IL-20 was responsive to hypoxia in vitro and in the ischemic stroke model and that up-regulation of IL-20 in the ischemic brain may contribute to brain injury PMID: 19342680
  • Data show that in lesional skin of psoriasis patients, highly elevated IL-20 levels strongly correlated with IL-22, and to a lesser extent, with IL-17A and TNF-alpha. PMID: 19830738

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    Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

    Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

    Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

    Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

    To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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