Recombinant Human Interleukin-12 Receptor Subunit Beta-2 (IL12RB2) Protein (His)

Name: Recombinant Human Interleukin-12 Receptor Subunit Beta-2 (IL12RB2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04520P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Interleukin-12 Receptor Subunit Beta-2 (IL12RB2) Protein (His) is produced by our Yeast expression system. This is a extracellular protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q99665
Target Symbol	IL12RB2
Synonyms	I12R2_HUMAN; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2; IL12 receptor beta 2; IL12R beta2; IL12RB2; Interleukin 12 receptor beta 2; Interleukin 12 receptor beta 2 chain; Interleukin-12 receptor subunit beta-2; RP11 102M16.1
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	KIDACKRGDVTVKPSHVILLGSTVNITCSLKPRQGCFHYSRRNKLILYKFDRRINFHHGHSLNSQVTGLPLGTTLFVCKLACINSDEIQICGAEIFVGVAPEQPQNLSCIQKGEQGTVACTWERGRDTHLYTEYTLQLSGPKNLTWQKQCKDIYCDYLDFGINLTPESPESNFTAKVTAVNSLGSSSSLPSTFTFLDIVRPLPPWDIRIKFQKASVSRCTLYWRDEGLVLLNRLRYRPSNSRLWNMVNVTKAKGRHDLLDLKPFTEYEFQISSKLHLYKGSWSDWSESLRAQTPEEEPTGMLDVWYMKRHIDYSRQQISLFWKNLSVSEARGKILHYQVTLQELTGGKAMTQNITGHTSWTTVIPRTGNWAVAVSAANSKGSSLPTRINIMNLCEAGLLAPRQVSANSEGMDNILVTWQPPRKDPSAVQEYVVEWRELHPGGDTQVPLNWLRSRPYNVSALISENIKSYICYEIRVYALSGDQGGCSSILGNSKHKAPLSGPHINAITEEKGSILISWNSIPVQEQMGCLLHYRIYWKERDSNSQPQLCEIPYRVSQNSHPINSLQPRVTYVLWMTALTAAGESSHGNEREFCLQGKAN
Expression Range	24-622aa
Protein Length	Extracellular Domain
Mol. Weight	69.7kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Type I cytokine receptor family, Type 2 subfamily
Database References	HGNC: 5972 OMIM: 601642 KEGG: hsa:3595 STRING: 9606.ENSP00000262345 UniGene: PMID: 29956791 Tumor cell differentiation is associated with TILs' expression of IL-12Rbeta2, and an IL-12Rbeta2(+) TIL ratio >/=35%) indicates favorable prognosis in LC PMID: 29103744 inhibition of the H3K27 demethylase JMJD3 in naive CD4 T cells demonstrates how critically important molecules required for T cell differentiation, such as JAK2 and IL12RB2, are regulated by H3K27me3. PMID: 28947543 this study identified susceptibility single nucleotide polymorphisms in IL12RB2 with Behcet's disease in Han Chinese PMID: 27464962 In ankylosing spondylitis, conditional analysis identified rs11209032 as the probable causal single-nucleotide polymorphism within a 1.14 kb putative enhancer between IL23R and IL12RB2. The rs11209032 single-nucleotide polymorphism downstream of IL23R forms part of an enhancer, allelic variation of which may influence Th1-cell numbers. PMID: 26916345 study confirmed the relationship of IL12RB2 polymorphisms with primary biliary cholangitis (PBC) susceptibility; provided evidence that IL12RB2 polymorphisms are associated with liver cirrhosis and an increased concentration of disease-specific anti-mitochondrial antibodies in sera of PBC patients PMID: 28299343 this study shows that single nucleotide polymorphisms of the IL23R-IL12RB2 region are associated with Behcet's disease in a Northern Chinese Han population PMID: 27660093 IL12RB2 polymorphisms correlate with risk of lung adenocarcinoma. PMID: 26547104 The effects of rs3762315 and rs3762316 single nucleotide polymorphisms in 5' flanking region on IL12RB2 transcription PMID: 26552659 Estrogen receptor beta 2 regulates IL12RB2 expression via p38 MAPK signaling and inhibits non-small-cell lung cancer proliferation and invasion. PMID: 25695486 IL12RB2 polymorphism is associated with systemic lupus erythematosus in the Chinese population. PMID: 25720506 results suggest the rs924080 risk A allele does not affect baseline expression of IL-12RB2, IL-23R, IFN-gamma and TNF-alpha but enhances IL-23R and TNF-alpha expression capacity in response to LPS stimulation; the A allele appears to enhance baseline IL-6 production PMID: 24434271 a differential regulation pattern for genes solely expressed in Th17 cells (IL17A and CCL20) compared to genes expressed in both Th17 and Th1 cells (IL23R and IL12RB2), where high levels of promoter methylation are correlated to near zero PMID: 22715389 Polymorphisms present in the promoter region of IL12RB2 may not be associated with susceptibility to leprosy or its clinical forms. PMID: 24486579 ERbeta2 and IL-12Rbeta5 had longer OS. PMID: 23677568 Data indicate that four of the IL-12Rbeta2 variants, N271Y, R313G, A604V and L808R showed reduced IL-12 responses compared to the wild type variant. PMID: 23911393 Association of IL12RB2 rs6679356 polymorphism with the age of type 1 diabetes mellitus onset suggests that this gene plays a role in defining the time of disease onset. PMID: 23152861 the -237 polymorphic site in the 5' promoter region of the IL-12Rbeta2 (SNP ID: rs11810249) gene associated with the AP-4 transcription motif GAGCTG PMID: 22509293 data clearly support the IL12RB2 genetic association with systemic sclerosis (SSc), and suggest a relevant role of the interleukin 12 signaling pathway in SSc pathogenesis. PMID: 22076442 Our results support a potential influence of the rs3790567 IL12RB2 polymorphism in the pathogenesis of giant cell arteritis. PMID: 21285166 significant interaction between the IL-12RB1 and IL-12RB2 genes contributes to a 4-fold increased risk for developing extrinsic atopic dermatitis PMID: 20060272 STATE OF ART REVIEW. IL-12Rb2 gene is not expressed in EBV-transformed normal B-lymphocytes and in Burkitt's lymphoma B-cell lines. PMID: 11940489 upregulated in Il-12 augmented T cell activation of mononclear leukocytes from HIV seropositive individuals PMID: 12804004 promoter activity is increased in case of the -465G allele, disrupting the intact GATA site PMID: 15140029 Study showed recombinant human interleukin-12 (rhIL-12) safe/well tolerated in healthy subjects given single doses (1 up to 8 ug) subcutaneously. Interferon-gamma and, signal transducer and activator of transcription may be useful biomarkers of rhIL-12. PMID: 15901746 Epigenetic silencing of IL-12Rbeta2 is a frequent event in lung cancers. Aberrant methylation of this gene seems to be a useful predictor of long-term outcome for adenocarcinoma of the lung. PMID: 17602269 frequencies of variant alleles were significantly higher in aggressive periodontitis patients as compared with healthy controls or chronic periodontitis patients PMID: 18353079 IL-12R beta 2 directly restrains multiple myeloma cell growth PMID: 18474725 These results suggest that these SNPs in IL12RB2 have differential effects on cellular activation of T cells and NK cells. PMID: 18771340 Single Nucleotide Polymorphism in interleukin 12 receptor beta 2 is associated with susceptibility to radiation dermatitis in breast cancer. PMID: 18927311 Our data show significant associations between primary biliary cirrhosis and common genetic variants at the HLA class II, IL12A, and IL12RB2 loci PMID: 19458352 Studies established the concept that the IL-12Rbeta2 gene is a gatekeeper from cancer. PMID: 19720917 In the generation of cell-mediated immunity against intracellular infection, the expression and function of the IL-12 receptor beta 2 chain correlate with the response of Th1-type cells to Mycobacterium leprae antigen. PMID: 11441083

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.