Recombinant Human Interferon Regulatory Factor 7 (IRF7) Protein (His)

Name: Recombinant Human Interferon Regulatory Factor 7 (IRF7) Protein (His)
Brand: Beta LifeScience
SKU: BLC-07136P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Interferon Regulatory Factor 7 (IRF7) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q92985
Target Symbol	IRF7
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MALAPERAAPRVLFGEWLLGEISSGCYEGLQWLDEARTCFRVPWKHFARKDLSEADARIFKAWAVARGRWPPSSRGGGPPPEAETAERAGWKTNFRCALRSTRRFVMLRDNSGDPADPHKVYALSRELCWREGPGTDQTEAEAPAAVPPPQGGPPGPFLAHTHAGLQAPGPLPAPAGDKGDLLLQAVQQSCLADHLLTASWGADPVPTKAPGEGQEGLPLTGACAGGPGLPAGELYGWAVETTPSPGPQPAALTTGEAAAPESPHQAEPYLSPSPSACTAVQEPSPGALDVTIMYKGRTVLQKVVGHPSCTFLYGPPDPAVRATDPQQVAFPSPAELPDQKQLRYTEELLRHVAPGLHLELRGPQLWARRMGKCKVYWEVGGPPGSASPSTPACLLPRNCDTPIFDFRVFFQELVEFRARQRRGSPRYTIYLGFGQDLSAGRPKEKSLVLVKLEPWLCRVHLEGTQREGVSSLDSSSLSLCLSSANSLYDDIECFLMELEQPA
Expression Range	1-503aa
Protein Length	Full Length
Mol. Weight	60.2 kDa
Research Area	Epigenetics And Nuclear Signaling
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
Subcellular Location	Nucleus. Cytoplasm. Note=The phosphorylated and active form accumulates selectively in the nucleus.
Protein Families	IRF family
Database References	HGNC: 6122 OMIM: 605047 KEGG: hsa:3665 STRING: 9606.ENSP00000329411 UniGene: PMID: 29222334 our results suggest that expression of IRF7 is one of the metastatic effectors of LMP1 signalling in Epstein-Barr virus-associated nasopharyngeal cancer PMID: 28712115 Hypomethylation of the IRF7 promoter might play a role in systemic sclerosis pathogenesis, probably through promoting the IRF7 expression in PBMCs of patients with SSc. PMID: 28952189 Results showed that INF-lambda serum concentration was increased in Alzheimer's disease (AD) and mild cognitive impairment (MCI) carrying the IFNL3 T allele compared to healthy controls (HC). Anti-HSV-1 Ab titers were higher in AD and MCI individuals carrying the IRF7 AA genotype compared to HC. IFNL3 rs12979860 and IRF7 rs6598008 polymorphisms may modulate immune responses against HSV-1 via their effect on the IFN-lam... PMID: 28984602 IRF7 plays a role in reducing bone metastasis of prostate cancer by IFN-beta-mediated NK activity. PMID: 27733217 The MYC is shown to be recruited to the IRF7 promoter region through interaction with nuclear receptor corepressor 2/histone deacetylase 3 for its repression. PMID: 27630164 S100A9 knockdown almost completely abrogated the effects of IRF7 deletion on granulocytic myeloid-derived suppressor cells (G-MDSC) development and tumor metastasis. IRF7 represents a novel regulator for G-MDSC development in cancer and may have predictive value for tumor progression. PMID: 28092673 the transcription factor NFATC3 binds to IRF7 and functions synergistically to enhance IRF7-mediated IFN expression in Plasmacytoid dendritic cells. PMID: 27697837 We show that IRF-7 siRNA knockdown enhanced LPS-induced IL-10 production in human monocyte-derived macrophages, and USP-18 overexpression attenuated LPS-induced production of IL-10 in RAW264.7 cells. Quantitative PCR confirmed upregulation of USP18, USP41, IL10, and IRF7. An independent cohort confirmed LPS induction of USP41 and IL10 genes PMID: 27434537 KSHV-encoded viral IRF4 interacts with the host IRF7 and inhibits interferon-alpha production. PMID: 28342865 The adaptor molecule RAIDD coordinates IKKepsilon and IRF7 interaction to ensure efficient expression of type I interferon. PMID: 27606466 Bcl6, by interacting with the co-factors NcoR2 and HDAC3, plays a pivotal role in controlling IRF7 induction and antiviral signaling priming. PMID: 26728228 Human IRF7 was shown to be essential for interferon type I-dependent protective immunity against primary influenza. (Review) PMID: 26761402 The IRF7 GG genotype associate with Cognitive Decline and Dementia. PMID: 25835418 IRF7 cleavage by the 3C protease of enterovirus D68 abrogated its capacity to activate type I interferon expression and limit virus replication. PMID: 26608321 Interferon regulatory factor 7 (IRF7) is a direct target of miR-762 and overexpression of miR-762 reduced expression of IRF7. PMID: 26597380 Data indicate that the type-I interferon master regulator gene interferon regulatory factor 7 (IRF7) is only hypomethylated in lupus patients with renal involvement. PMID: 26005050 our results indicate that IRF7 promotes glioma cell invasion and both chemoresistance and radioresistance through AGO2 inhibition PMID: 25680411 The interaction between AIP and IRF7 is enhanced upon virus infection, and AIP potently inhibits IRF7-induced type I IFN (IFN-alpha/beta) production. PMID: 25911105 Authors found that knockdown of IRF7 leads to growth inhibition of Epstein-Barr virus-transformed cells, and restoration of IRF7 by exogenous plasmid correlates with growth recovery of the viral transformed cells. PMID: 25300801 In TSC2-deficient angiomyolipoma patient cells, IRF7 is a pivotal factor in the Rheb/mTOR pathway. PMID: 25476905 These findings suggest that IRF7-dependent amplification of type I and III IFNs is required for protection against primary infection by influenza virus in humans. PMID: 25814066 Authors conclude that paramyxoviruses trigger the DNA damage response, a pathway required for MSK1 activation of phospho Ser 276 RelA formation to trigger the IRF7-RIG-I amplification loop necessary for mucosal interferon production. PMID: 25520509 phosphorylation-mediated IRF7 transactivation is controlled by a tripod-helix structure. PMID: 25225665 Data indicate that knockdown of IRF-7 expression almost completely diminished the enhancing effect of TLR9 signaling on Foxp3 expression, suggesting that IRF-7 is a downstream molecule of TLR9 signaling. PMID: 23490285 In over 800 breast cancer patients, high expression of Irf7-regulated genes in primary tumors was associated with prolonged bone metastasis-free survival. PMID: 22820642 Tat interaction with the 2 MAPKK and IRF7 promoters in HIV-1-infected cells and the resulting persistent activation of interferon-stimulated genes, which include inflammatory cytokines and chemokines, can contribute to the increased immune activation. PMID: 23535064 In the present study, the authors report that enterovirus 71 downregulates IRF7 through the 3C protein, which inhibits the function of IRF7. PMID: 23175366 The major allele of a nonsynonymous polymorphism, rs1131665 (412Q) in IRF7, confers elevated activation of IRF-7 and predisposes to the development of systemic lupus erythematosus in multiple ethnic groups. PMID: 21360504 None of the IRF7 polymorphisms was associated with systemic lupus erythematosus. PMID: 22433914 phosphorylation of IRF7 on Ser477 and Ser479 by IKKepsilon or TBK1 is inhibited by KSHV ORF45 PMID: 22787218 discuss the association of IRF7 and SLE based on recent understandings to render more information about the mechanisms of IRF7 might perform in PMID: 22455868 Reconstruction of the wiring diagram of the modules revealed the presence of hyperconnected hub nodes, most notably interferon regulatory factor 7, which was identified as a major hub linking interferon-mediated antiviral responses. PMID: 22112518 IRF7 single nucleotide polymorphism rs1061501 TT genotype and T allele are enriched in Taiwanese patients with systemic lupus erythematosus (SLE) and seem to be associated with an increased risk of developing SLE. PMID: 21632682 Vesicular stomatitis Indiana virus-activated IRF7 upregulates expression of the BST2 gene independently of interferon signaling. PMID: 22301143 TRAF6 can regulate HIV-1 production and expression of IRF7 promotes HIV-1 replication. PMID: 22140520 IRF7 region is an anticentromere autoantibody propensity locus in systemic sclerosis PMID: 21926187 Vaccinia virus protein C6 inhibits the activation of IRF7 by TBK1- and IKKepsilon-dependent pathways. PMID: 21931555 The ubiquitin E3 ligase activity of tripartite motif-containing (TRIM)28 protein is specific to IRF7. PMID: 21940674 Cigarette smoke suppresses key plasmacytoid dendritic cells functions upon viral infection by a mechanism that involves downregulation of TLR7 expression and decreased activation of IRF-7. PMID: 21435390 Study did not find signi fi cant relationships between rs4963128 or rs2246614 of IRF7/KIAA1542 and the risk of systemic lupus erythematosus, differing from a previous study of European women PMID: 21167895 analysis of IFN-stimulated response elements (ISREs) that bind to both the IFN-stimulated gene factor 3 (ISGF3) as well as to IFN response factor 7 (IRF7) PMID: 20943654 ORF45 may maintain the IRF-7 molecule in the closed form and prevent it from being activated in response to viral infection PMID: 20980251 These findings demonstrate that the lysine residues of IRF-7 play important roles in mediating IFN synthesis and modulating viral lytic replication. PMID: 20844090 IRF-7-induced HPV8 transcription in primary keterinocytes. PMID: 20980500 interferon regulatory factor 7C has dual roles in Epstein-Barr virus-mediated lymphocyte transformation PMID: 20209099 HCV infection enhances STAT1 expression but impairs nuclear translocation of IRF-7 and its downstream molecules. These impairments in the IFN-alpha signaling pathway may, in part, be responsible for establishment of chronic HCV infection. PMID: 20810735 Results suggest that Ro52-mediated ubiquitination promotes the degradation of IRF7 following TLR7 and TLR9 stimulation. PMID: 20668674 EBV infection correlated with a blockage in the activation of JAK/STAT pathway members and affected the level of phosphorylated IFN regulatory factor 7 (IRF7). PMID: 20689596 Smokers after infection with influenza is associated with reduced expression of IRF7 in nasal epithelial cells. PMID: 19880818

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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