Recombinant Human Interferon alpha 10 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2980
Recombinant Human Interferon alpha 10 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2980
Collections: Other recombinant proteins, Recombinant proteins
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Product Overview
Tag | Fc |
Host Species | Human |
Accession | P01566 |
Synonym | IFN-alphaC, MGC119878, MGC119879 |
Background | Interferon alpha-1 (IFNA1) is a member of the interferon family. Interferons belong to the group of the regulatory glycoproteins, of low molecular mass. They are the products of infected cell-genome, but not virus, as a consequence of the cause answer by different inductors. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs have other functions: they activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they increase the ability of uninfected host cells to resist new infection by virus. Certain host symptoms, such as aching muscles and fever, are related to the production of IFNs during infection. Human IFN are divided on the sequence of amino-acids into three groups: Alpha, Beta and Gamma interferons. |
Description | A DNA sequence encoding the human IFNA10 (P01566) (Cys 24-Asp 189) was fused with the Fc region of human IgG1 at the N-terminus. |
Source | HEK293 |
Predicted N Terminal | Glu |
AA Sequence | Cys 24-Asp 189 |
Molecular Weight | The recombinant human IFNA10/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 427 a.a. with a predicted molecular mass of 48 kDa. AsrhIFNA10/Fc monomer migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions. |
Purity | >85% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitisvirus (VSV).The ED50 for this effect is 20-80 pg/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. |
Subcellular Location | Secreted. |
Protein Families | Alpha/beta interferon family |
Database References |
Gene Functions References
- Heterozygous IFNA10 variants is a cause of impaired function and CD-susceptibility genes in Chinese individuals from multiple center based study. PMID: 26000985
- We did not reproduce the associations previously noted with the TNF, IFNA10, IFNA17, and IFNG genes with pulmonary sarcoidosis. PMID: 20722470