Recombinant Human Integrin Alpha-2 (ITGA2) Protein (His-SUMO)

Name: Recombinant Human Integrin Alpha-2 (ITGA2) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-09290P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Integrin Alpha-2 (ITGA2) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P17301
Target Symbol	ITGA2
Synonyms	BR; CD 49b; CD49 antigen like family member B; CD49 antigen-like family member B; CD49b; CD49b antigen; Collagen receptor; DX5; Glycoprotein Ia deficiency included; GP Ia; GP Ia deficiency; included; GPIa; HPA 5 included; HPA5 included; Human platelet alloantigen system 5; Integrin alpha 2; Integrin alpha II; Integrin alpha-2; Integrin; alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor); ITA2_HUMAN; ITGA2; Platelet alloantigen Br(a); included; Platelet antigen Br; Platelet glycoprotein GPIa; Platelet glycoprotein Ia; Platelet glycoprotein Ia/IIa; Platelet membrane glycoprotein Ia; Platelet receptor for collagen; deficiency of; included; Very late activation protein 2 receptor alpha 2 subunit; VLA 2 alpha chain; VLA 2; VLA 2 subunit alpha; VLA-2 subunit alpha; VLA2; VLA2 receptor alpha 2 subunit; VLAA2
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	WDAVKNFLEKFVQGLDIGPTKTQVGLIQYANNPRVVFNLNTYKTKEEMIVATSQTSQYGGDLTNTFGAIQYARKYAYSAASGGRRSATKVMVVVTDGESHDGSMLKAVIDQCNHDNILRFGIAVLGYLNRNALDTKNLIKEIKAIASIPTERYFFNVSDEAALLEKAGTLGEQIFSIE
Expression Range	188-365aa
Protein Length	Partial
Mol. Weight	35.6kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A.; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Integrin alpha chain family
Database References	HGNC: 6137 OMIM: 192974 KEGG: hsa:3673 STRING: 9606.ENSP00000296585 UniGene: Hs.482077

Gene Functions References

Interleukin-4 induces a CD44high /CD49bhigh PC3 subpopulation with tumor-initiating characteristics PMID: 29236307
Carriage of a combination of mutant alleles in multiple genes including ITGB3+CYP2C19*2 or CYP2C19*2 + ITGA2 or CYP2C19*2 are possible predictors of CVE in patients after CABG PMID: 29461866
Studied the association between integrin subunit alpha 2 (GPIa) and prostaglandin-endoperoxide synthase 2 (COX-2) genetic polymorphisms in Chinese ishemic stroke patients with or without aspirin resistance. PMID: 28948649
GP Ia C807T polymorphism may contribute to ischemic stroke development in the Chinese population (meta-analysis). PMID: 29208186
The authors identified a cell surface marker, ITGA2, which marks a proliferative trophoblast progenitor compartment in the first trimester placenta that is regulated by NOTCH signalling and exhibits unique expression characteristics. PMID: 29540503
discovered that OLFM4, CD11b, and ITGA2 are proteins that are overexpressed in both primary colon tumors and liver metastasis. These are colon cancer biomarkers with autoantigenic properties. PMID: 28669815
This study demonstrated that the transactivation of FOXL2 driven by interactions between HMGA2 and pRb might exert critical effects on the metastases and EMT of chemoresistant gastric cancer. Blocking the HMGA2-FOXL2-ITGA2 pathway could serve as a new strategy for gastric cancer treatment. PMID: 28119367
The GpIa-C807T and GpIIIa-PlA1/PlA2 polymorphisms and more pronouncedly their combination are associated with increased risk of spontaneous abortions. PMID: 27405521
The allele combination of CGC from hOGG1, ITGA2 and XPD polymorphisms was significantly associated with increased odds of nasopharyngeal carcinoma. PMID: 29121049
blood platelets have a role in amyloid-beta aggregation in cerebral vessels through integrin alphaIIbbeta3-induced outside-in signaling and clusterin release PMID: 27221710
Exposure to magnesium ions facilitated hBMSC proliferation via integrin alpha2 and alpha3 expression and partly promoted differentiation into osteoblasts via the alteration of ALP expression and activity PMID: 24616281
The synonymous common variant, GPIA rs1126643, increases the long-term adverse events risk of CABG by augmenting GPIa protein expression and enhancing platelet aggregation function. PMID: 27881421
we found a significant cumulative contribution of the genetic heterogeneity of glycoproteins Ia and IIIa and platelet-endothelial cell adhesion molecule-1 and P-Selectin genes in the risk of recurrent IVF-ET failures. The coexistence of these SNPs was associated with increased IVF-ET failure risk and the more polymorphic alleles or genotypes were present the higher the risk of IVF-ET failure, especially for younger women PMID: 28388872
Meta-analysis found that glycoprotein Ia C807T T allele or the TT genotype, the Ser-allele of HPA-3 and B allele of glycoprotein Ibalpha variable number tandem repeat polymorphisms were associated with increased risk for ischemic stroke. PMID: 28004990
Data indicate no association of maternal or fetal ITGA2 C807T SNP, ITGB3 T1565C SNP, PECAM1 CTG - GTG and SELP A/C polymorphisms with fetal growth restriction (FGR). PMID: 28358707
The most common HPA genotypes among Saudis were HPA-1 a + b- (75%), HPA-2 a + b- (62%), HPA-3 a + b- (51.5%), HPA-4 a + b- (99%), HPA-5 a + b- (76.5%), HPA-6 a + b- (100%) and HPA-15 a + b + (50%). The prevalent allele among the HPA systems was (a), except in the HPA-15 system where the (b) allele was found in 52% of the subjects. PMID: 27019315
A sulfonamide-type small-molecule inhibitor of alpha2beta1 integrin decreased average platelet adhesion in individuals with the C/T807T genotype but not in those harboring C807C. PMID: 26556301
Platelet GP Ia 807C/T polymorphism could be a protective factor of for cerebral hemorrhage in the Chinese population. PMID: 26134877
high miR-128 expression suppressed osteosarcoma cell migration, invasion, and EMT development through targeting ITGA2, which may be recommended as a therapeutic target for osteosarcoma. PMID: 26700675
Study identified a novel RANKL function in the promotion of cell adhesion to type I collagen through the RANKL-NF-kappa B-integrin alpha 2 pathway and found integrin alpha2 and RANKL up-regulated in non small cell lung carcinoma. PMID: 27009236
Recently, new clinical observations of genetic diseases provided evidence bringing new data on the role of alphaIIbbeta3 integrin in defective megakaryopoiesis. [review] PMID: 27011248
High ITGA2 expression is associated with cervical cancer. PMID: 26758421
PDI-mediated disulfide bond exchange plays a pivotal role in the post-ligation phase of alphaIIbbeta3-mediated adhesion to fibrinogen, while this step in alphavbeta3-mediated adhesion is independent of disulfide exchange PMID: 26514956
Our results indicate that GpIa-807T and GpIIIa-PlA2 may be susceptibility alleles for in vitro fertilization implantation failure. PMID: 25643034
PFL-mediated downregulation of integrin and EGFR contributes to the inhibition of tumor growth in vitro and in vivo. This novel anti-cancer mechanism of PFL suggests that this lectin would be useful as an anti-cancer drug or an adjuvant for other drugs. PMID: 26850110
HRG binds to alpha2 integrin through low-affinity interactions in a heparin sulfate-independent manner, thereby blocking endothelial cells adhesion to collagen I. PMID: 26051322
The Glycoprotein Ia C807T polymorphism might be a risk factor for the development and relapse of Acute Coronary Syndrome PMID: 26304618
Data indicate that in recurrent spontaneous abortion patients, the expression levels of CD49b and LAG-3 (CD223) on CD14(+) mononuclear cells and the plasma level of transforming growth factor beta (TGF-beta) decreased obviously compared with normal females. PMID: 26927558
Integrin alpha 2 and alpha 4 Yxxphi motifs interact with the endocytic clathrin adaptor, AP2 complex. PMID: 26779610
Epigenetic silencing of ITGA2 by MiR-373 promotes cell migration in breast cancer. PMID: 26258411
A highly bone metastatic RANKL-overexpressing prostate cancer cell line, LNCaP(RANKL)used as a model to study increased adhesion of prostate cancer (PCa) cells to collagens and found 3-D suspension culture and in vivo PCa tumor growth restore AR through downregulation of AP-4, enhancing integrin alpha2 expression and adhesion to ColI which is rich in bone matrices. PMID: 25200184
diabetic retinopathy is associated with ITGA2 and ITGB3 PlA1/A2 polymorphisms (Meta-Analysis) PMID: 24979111
This study shows that osteoblastic differentiation is improved on 3D scaffolds with submicron-scale texture and is mediated by integrin alpha2beta1. PMID: 25203434
Current review discusses the mechanisms by which integrin is activated and subsequently deactivated by focusing on its structure-function relationship PMID: 26152212
Polymorphism of HPA- 5 is probably a risk factor for carotid atherosclerosis complicating T2DM. PMID: 25966234
blockade of alpha2beta1 integrin with a neutralizing mAb inhibited IL-7-induced bone loss and osteoclast numbers by reducing Th17 cell numbers in the bone marrow PMID: 26408663
EZH2-silenced cells the signaling activity of the de-repressed ITGalpha2 is able to increase cofilin phosphorylation, which in turn reduces cell migration. PMID: 25549357
The Integrin alpha2 2 gene C807T polymorphism may be a susceptible predictor of the risk of ischemic stroke. PMID: 24397542
CD34 and CD49b are cell surface markers that can be used to enrich a subpopulation of leiomyoma cells possessing stem/progenitor cell properties. PMID: 25658015
alpha2beta1 integrin gene expression levels were increased significantly in keloid fibroblasts under tension compared to normal fibroblasts under tension. Knockdown of Integrin A2B1 reduced cell proliferation in fibroblasts under tension. PMID: 25046655
Carbohydrate-to-carbohydrate interactions between alpha2,3-linked sialic acids on alpha2 integrin subunits and asialo-GM1 underlie the bone metastatic behaviour of LNCAP-derivative C4-2B prostate cancer cells. PMID: 25137483
The alphaII b tail sequences distal of GFFKR participate in talin-mediated inside-out alphaII b beta3 activation through its steric clashes with beta3 -bound talin PMID: 24837519
The alpha2beta1 integrin mediates T-acute lymphoblastic leukemia cell migration through collagen in ERK and p38 MAPKs-dependent manner PMID: 24880062
These results reveal betaig-h3 enhances the metastasis potentials of human osteosarcoma cells via integrin alpha2beta1-mediated PI3K/AKT signal pathways. PMID: 24595049
ITGA2 is downregulated 39-fold by silencing of laeverin in trophoblasts, reducing cell migration and invasion. PMID: 24959655
Molecular modeling indicated that galactosylation occurred on the periphery of alpha2beta1 integrin interaction with alpha1(IV)382-393 but right in the middle of alpha3beta1 integrin interaction with alpha1(IV)531-543 PMID: 24958723
ITGA2 +807 gene polymorphism is not associated with susceptibility to CsA-induced GO. PMID: 24581850
glycoprotein Ia C807T polymorphism is associated with idiopathic sudden hearing loss. PMID: 24466284
It plays a role in regulation of leukocyte migration to a site of inflammation. PMID: 24850451
Usage of internal N-acetylneuraminic acids and alpha2beta1 integrin by Wa rotavirus was at least partially additive. PMID: 24501414

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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