Recombinant Human IL-16 Protein

Name: Recombinant Human IL-16 Protein
Brand: Beta LifeScience
SKU: BL-1738NP
Availability: InStock

BL-1738NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

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Product Overview

Description	Recombinant Human Interleukin-16 is produced by our E.coli expression system and the target gene encoding Met1-Ser130 is expressed.
Accession	AAC12732.1
Synonym	Pro-Interleukin-16; Interleukin-16; IL-16; Lymphocyte Chemoattractant Factor; LCF; IL16
Gene Background	Interleukin-16 (IL-16) is a CD8+ T cell-derived cytokine that induces chemotaxis of CD4+ T cells and CD4+ monocytes and eosinophils. Analysis by gel filtration suggests that, under physiological conditions, human IL-16 exists predominantly as a noncovalently linked multimer, but that some IL-16 may exist as a monomer. However, only the multimeric form appears to possess chemotactic activity, suggesting that receptor cross-linking may be required for activity. IL-16 also induces expression of IL-2 receptor (IL-2R) and MHC class II molecules on CD4+ T cells. Human and murine IL-16 show significant cross-species reactivity.
Molecular Mass	13.4 KDa
Apmol Mass	16-20 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.; may act as a scaffolding protein that anchors ion channels in the membrane.; Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
Subcellular Location	[Interleukin-16]: Secreted.; [Isoform 1]: Cytoplasm.; [Isoform 3]: Cytoplasm. Nucleus.
Database References	HGNC: 5980 OMIM: 603035 KEGG: hsa:3603 STRING: 9606.ENSP00000302935 UniGene: PMID: 29328375 There was a decreased frequency of IL16 rs4778889 CC genotype carriers among women with gestational diabetes mellitus, however, there was no significant difference in the distribution of alleles. PMID: 28580570 Serum IL-6, serum YKL-40, and plasma VEGF were significantly correlated with disease activity at baseline in early rheumatoid arthritis. PMID: 29336711 serum IL-16 level may not only serve as a useful diagnostic biomarker for patients with GC, but also may predict cancer recurrence and patient prognosis. PMID: 28990054 Association of interleukin 16 gene polymorphisms and plasma IL16 level with osteosarcoma risk. PMID: 27703190 Results suggest that IL16 genotype predict higher risk of death and progression, respectively, in NSCLC patients PMID: 28807247 rs11556218 T/G genotype associated with increased risk for development of osteoporosis in Chinese postmenopausal women PMID: 27984748 Data indicate an association between interleukin-16 (IL-16) rs4072111 and rs1131445 with progression to the severe stages (III-IV) of endometriosis. PMID: 27484651 Long-term exposure to tobacco smoke does not markedly alter extracellular concentrations of IL-16 protein in blood. However, it does decrease the intracellular IL-16 concentrations in blood NK cells. PMID: 27695312 data revealed an association between circulating IL-16 and severity of psoriasis which indicates that this cytokine could serve as a potential marker of disease activity. However, further investigations are required PMID: 27788245 Structure of a Potential Therapeutic Antibody Bound to Interleukin-16 PMID: 27231345 No significant differences were observed in rs11556218 genotype frequencies between the study groups. In conclusion, the results of our study reveal an association between the IL-16 rs4778889 polymorphism and heightened risk of renal cell cancer . PMID: 27323152 We found that the rs1131445 T/C and rs4072111 T/C variants of IL-16 were significantly associated with increased risk of gastric cancer in Iranian population. PMID: 26346169 the results of our study suggested an association between the IL-16 rs4778889 polymorphism and an elevated risk of renal cell carcinoma PMID: 26823871 We observed highly significant reductions in the concentration of circulating interleukin (IL)-16, IL-7, and Vascular Endothelial Growth Factor A (VEGF-A) in encephalomyelitis/chronic fatigue syndrome patients. PMID: 26615570 IL-16 rs1131445 C/T polymorphism is related to the susceptibility to cancer in Asians (meta-analysis) PMID: 26842762 The IL16 rs4072111 polymorphism may be associated with susceptibility to AD and the T allele may have a protective role in the progression of AD in an Iranian population. PMID: 26386715 intrathyroidal production of IL-14 and IL-16 associated with the pathogenesis of autoimmune thyroid disease PMID: 25940130 the lack of a relationship between genotype and serum levels of IL-16 seems to suggest a more complex regulation of this cytokine at the post-translational level and/or the interplay with other cytokines. PMID: 26154181 The results suggested that the variants in IL-16 gene rs11556218 site were associated with a decreased knee osteoarthritis risk after adjusting for age, sex, BMI, and smoking and drinking status. PMID: 25954818 Data suggest that thymic stromal lymphopoietin (TSLP) and interleukin-16 (IL-16), expressed at early stages of disease, function to recruit malignant T cells to the skin. PMID: 24794807 The production of IL-16 contributed to lung damage as neutralization of IL-16 enhanced S. aureus clearance and resulted in diminished lung pathology in S. aureus pneumonia. PMID: 24736233 The findings suggest that the IL-16 rs 11556218 polymorphism may be used as a susceptibility marker for glioma. PMID: 25166752 IL-16 rs11556218 T/G was associated with a significant increased risk of cancer. PMID: 24969906 The rs11556218 T/G polymorphism of the IL-16 gene was significantly associated with elevated cancer risk in Asian populations. [meta-analysis] PMID: 24328527 IL16 polymorphisms may play a role in the pathophysiology of alopecia areata or in the expression of phenotypes in Koreans. PMID: 24320753 IL-16 rs4778889 T/C polymorphism is not associated with cancer. PMID: 24568499 The IL-16 SNP rs8028364 was found to be associated with Graves' disease(GD) when compared with the control subjects. PMID: 24694201 An association between decreased plasma IL-16 and emphysema susceptibility. PMID: 24138069 Rs4778889 CC and rs11556218 GG genotype was significantly associated with 1.97 and 1.84-fold increased risk of non-cardia gastric cancer. PMID: 24175812 the IL-16 polymorphism may be related to the etiology of ischemic stroke in the Chinese population PMID: 24288444 This study showed that tissue expression and serum levels of IL-16 increase in association with malignant ovarian tumor development and progression. PMID: 24380743 IL-16 plays an important role in the proliferation and apoptosis of MT-4 cells PMID: 24200060 Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction. PMID: 23894370 The IL-16 SNPs rs8034928 and rs11556218 are associated with the coronary artery disease risk in the Chinese population. PMID: 23881440 It is feasible to predict preterm birth by measuring the amniotic fluid levels of IL-16 especially for the pregnant women requiring genetic amniocentesis during early second trimester. PMID: 23020666 Authors identified three independent loci within IL16 that were associated with prostate cancer risk. SNP expression quantitative trait loci analyses revealed that rs7175701 is predicted to influence the expression of IL16 and other cancer-related genes. PMID: 22923025 study identified IL-16 as a critical tristetraprolin-regulated chemotactic factor that contributes to monocytes/macrophages migration PMID: 23241166 a significant relationship exists between the miRNA-binding site polymorphism of the IL-16 gene and colorectal cancer risk in the Iranian population PMID: 22939228 IL-16 was strongly overexpressed in the bone marrow of myeloma patients compared with healthy donors. IL-16 is an important growth-promoting factor in multiple myeloma. PMID: 22745469 IL-16 rs11556218 G/T polymorphism is significantly associated with the risk of coronary artery disease in the Chinese Han population. PMID: 21703255 Data show that IL16 single nucleotide polymorphisms are associated with chronic hepatitis B virus infection and hepatocellular carcinoma. PMID: 22019522 Serum IL-16 levels of atopic dermatitis patients correlate to some extent with sensitization. PMID: 21447961 Our results demonstrate that loss of intracellular IL-16 in peripheral blood T cells corresponds to lymphoma stage in Sezary Syndrome patients and orrelates with loss of surface CD26. PMID: 20878214 TcdA and TcdB downregulate IL-16 gene expression via inhibition of Rho GTPases.(TcdA and TcdB from C. difficile strain VPI 10463) PMID: 21267712 The TG/GG genotypes of rs11556218 T/G were associated with a significantly increased risk of coronary artery disease as compared with the TT genotype. PMID: 21214401 Ability of IL-16 homolog from leech Hirudo medicinalis to recruit microglial cells to sites of nervous system injury suggests its in the crosstalk between neurons and microglia in the leech central nervous system repair. PMID: 20578037 Tobacco smoke depletes preformed intracellular IL-16 protein, inhibits its de novo synthesis, and distorts key cellular functions in human CD8(+) cells. PMID: 21036918 the rs4778889 T/C polymorphism of the IL-16 gene may be associated with risk of endometriosis PMID: 20662556 IL-16 -295 T>C polymorphism is significantly associated with a higher risk of developing RCC in Chinese population. PMID: 20529140

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human IL-16 Protein

Recombinant Human IL-16 Protein

Product Overview

Target Details

FAQs