Recombinant Human IL-10RA Protein (C-6His)

Name: Recombinant Human IL-10RA Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-2101NP
Availability: InStock

BL-2101NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

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Product Overview

Description	Recombinant Human Interleukin-10 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding His22-Asn235 is expressed with a 6His tag at the C-terminus.
Accession	Q13651
Synonym	Interleukin-10 receptor subunit alpha; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; CD210; IL10RA
Gene Background	Interleukin-10 Receptor alpha (IL-10Rα) is a transmembrane glycoprotein member of the class II cytokine receptor family. Mature human IL-10 Rα consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain. Within the ECD, human IL-10 Rα shares 59% aa sequence identity with mouse and rat IL-10Rα. IL-10 Rα is required for mediating the effects of IL-10,a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer. IL-10Rα is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 Rα, resulting in the recruitment of IL-10 Rβ. Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection. Polymorphisms of human IL-10 Rα may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL10.
Molecular Mass	25.2 KDa
Apmol Mass	38-59 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Cytoplasm.
Protein Families	Type II cytokine receptor family
Database References	HGNC: 5964 OMIM: 146933 KEGG: hsa:3587 STRING: 9606.ENSP00000227752 UniGene: PMID: 28644354 We identified 32 compound heterozygous mutations and 9 homozygous mutations in IL10 receptor subunit alpha and 1 homozygous mutation in IL10 receptor subunit beta. Among these mutations, 10 novel mutations were identified, and 6 pathogenic mutations had been previously described. In patients with IL10 receptor subunit alpha mutations, c.301C>T (p.R101RW) and c.537 G>A (p.T179T) were the most common mutations. PMID: 28267044 these findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA-dependent repression of claudin-2 PMID: 28893958 Expression of IL10R subunits within the leukocyte population (CD45(+) cells) was significantly higher in primary brain tumors than in metastases. PMID: 28982901 This study showed that lack of association with schizophrenia was detected for IL10 and IL10RA single polymorphisms and haplotypes. PMID: 27397081 The IL-10RA rs9610 A allele was increased in rheumatoid arthritis (RA) patient group compared with control subjects. Interestingly, significant differences were detected both in the allele and genotype frequencies of rs9610 between anti-CCP (anti-cyclic citrullinated peptide) positive patients and anti-CCP negative patients. The findings suggest that IL-10RA rs9610 polymorphism might contribute to RA susceptibility. PMID: 27796662 These results suggest that S138G loss-of-function polymorphism of the IL-10R1 may be important risk factor in increasing susceptibility to multiple sclerosis. PMID: 28225209 Results reveal the structure of box1 from class II cytokine receptors IFNLR1 and IL10RA bound to the FERM-SH2 domain of human JAK1, identifying a consensus motif for JAK1 interaction. PMID: 27133025 a JAK2 Inhibitor Suppresses a BCL6-dependent IL10RA/JAK2/STAT3 Pathway in High Grade B-cell Lymphoma. PMID: 27268052 genetic association study in cohort of infants/children: Data suggest perianal fistulas are exhibited in patients with very early-onset inflammatory bowel disease with IL10 receptor mutations (IL10RA/B); prognosis and response to treatment are poor. PMID: 25373860 IL-10Ralpha expression is post-transcriptionally regulated by miR-15a, miR-185, and miR-211 in melanoma PMID: 26631117 SNP3 polymorphism is associated with myocardial infarction; may generate some conformational rearrangements of the IL-10R1 receptor domain which affects IL-10 complex binding and alters its downstream signal PMID: 24566517 High IL10RA expression is associated with diffuse large B-cell lymphoma. PMID: 25733167 Inflammatory bowel disease (IBD) in infancy is phenotypically and genetically different disease entity from adult-onset or older child-onset IBD. It has a strong association with IL-10 receptor gene. PMID: 24785691 IL10R1 loss-of-function A536/S138G polymorphism may contribute to recurrent pregnancy loss pathogenesis PMID: 24689510 Very early onset inflammatory bowel disease associated with aberrant trafficking of IL-10R1 and cure by T cell replete haploidentical bone marrow transplantation. PMID: 24519095 Mutations in the IL10RA gene is associated with ulcerative colitis. PMID: 24216686 Case Report: pediatric ulcerative colitis with a novel point mutation within the IL10RA promoter (the -413G->T), inherited from his mother. PMID: 24379584 This report confirms the genetic defect of IL-10RA in neonatal-onset inflammatory bowel disease. PMID: 23839161 IFN-gamma selectively induced the expression of IL-10R1 on intestinal epithelia, predominantly on the apical membrane of polarized epithelial cells. PMID: 24367025 A mutation in TLR4 (rs4986790) and IL10RA (rs22291130) was significantly associated with Mycobacterium avium subspecies paratuberculosis-positive Crohn's disease patients. PMID: 23455702 5 patients with an IL-10R1 or IL-10R2 deficiency developed B-cell non-Hodgkin lymphoma between the ages of 5 and 6 years (which was recurrent in 1 patient). PMID: 24089328 IL10RA polymorphisms are associated with ulcerative colitis. PMID: 22550014 In the Chinese Han population, missense SNPs within the exons of the IL-10R1 gene do not contribute to the development of systemic lupus erythematosus PMID: 22652629 The results suggest that genetic polymorphisms in TNF and IL10RA genes may modify the association between blood transfusion and NHL risk. PMID: 22649007 IL-10R1 is a novel substrate of betaTrCP-containing ubiquitin E3 ligase, a novel negative regulatory mechanism that may potentially affect IL-10 function PMID: 22087322 We found an IL10R variant, which may be associated with a decreased response to the cytokine in one patient. PMID: 22155628 IL-10R1 S138G loss-of-function polymorphism is associated with extrapulmonary tuberculosis risk development in Tunisia. PMID: 21553229 The haplotype -185/-116 of IL10 receptor alpha in combination with the haplotype -754/-750 of IL10 receptor beta contributed towards mild malaria. PMID: 21814839 IL10R1-G330R does not alter surface expression but duration of STAT phosphorylation, indicating that the position of G330 is important in stabilizing the STAT signal. PMID: 21654841 linkage disequilibrium (LD) blocks were formed in IL10 and IL10RA PMID: 21532858 IL-10R1 expression on CD4+ T cells and signalling in PBMCs were down-regulated in lupus nephritis (LN) patients, indicating that IL-10 and its receptor may have a special role in LN pathogenesis PMID: 21635228 Besides overall immune health, host genetic factors influence mortality, retinitis progression, and retinal detachment in patients with AIDS and CMV retinitis that are receiving HAART. PMID: 21396623 in the Lebanese population, the loss-of-function allele IL-10R1-S138G (SNP3) is unlikely to provide a protective effect against ulcerative colitis and that both IL-10R1 variants do not correlate with inflammatory bowel disease PMID: 20186944 G carriers for the -536AG IL-10R1 gene polymorphism had higher systolic and diastolic pressures, and IL-10 levels; and obese G carriers had an increased waist-to-hip ratio. PMID: 19798061 cmvIL-10 forms a disulfide-linked homodimer that binds two sIL-10R1 molecules. PMID: 12093920 IL-10 receptor stimulated the rapid translocation of IL-10E1 to the cell nucleus and the activation of TIMP-1 expression in primary human prostate tumor cell lines. PMID: 12496489 Abnormal interleukin 10Ralpha expression contributes to the maintenance of elevated cyclooxygenase-2 in non-small cell lung cancer cells. PMID: 12591723 This investigation reveals three previously unrecognized polymorphisms of IL-10R1 (SNP3, SNP4, and SNP5), two of which result in an amino acid substitution; substitution in S138G variant may interfere with binding of IL-10 to IL-10R1. PMID: 12759436 Homozygosity of the IL-10R1 G330R allele is associated with schizophrenia and may contribute to the expression of disease phenotype in susceptible individuals. PMID: 17066477 Genetic variation in IL-10RA/IL-22 genes may play a modulatory role in the outcome of hepatitis C infection. PMID: 17845543 The IL-4R Ile50/Ile50 and IL-10R2 G520/G520 and G520/A520 genotypes were shown to determine the susceptibility to SLE (systemic lupus erythematosus)in a Chinese population PMID: 17988330 The peripheral blood neutrophils of septic patients constitutively display abundant levels of surface IL-10R1. PMID: 18308712 IL-10R is associated with the progression of the renal cell carcinoma PMID: 18344594 Data demonstrate a significant relation between cervical concentrations of IL-10 and single nucleotide polymorphisms in the IL-10 receptor alpha and beta genes. PMID: 18674658 The IL-10R1 S138G loss-of-function allele and ulcerative colitis are reported. PMID: 18800073 a variant rs17121510 in the interleukin-10 receptor antagonist (IL-10RA) gene for allele (p = 0.01) and genotype (p = 3.34x10(-4)) may have a role in preterm birth PMID: 18818748 IL-10R1 variants differentially reduce the signaling activity of cmvIL-10 PMID: 19016528 Mutations in genes encoding the IL10R subunit proteins were found in patients with early-onset enterocolitis, involving hyperinflammatory immune responses in the intestine. PMID: 19890111 The capacity of neutrophils to respond to IL-10, as assessed by Stat3 tyrosine phosphorylation, SOCS-3 expression, and modulation of cytokine production, is dependent on the level of expression of IL-10R1. PMID: 11490020

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human IL-10RA Protein (C-6His)

Recombinant Human IL-10RA Protein (C-6His)

Product Overview

Target Details

FAQs