Recombinant Human IL-7 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0041
Recombinant Human IL-7 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at ~17 kDa and purity greater than 98%.
Recombinant Human IL-7 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at ~17 kDa and purity greater than 98%.

Recombinant Human IL-7 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0041
/
Size

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Product Overview

Product Name Recombinant Human IL-7 Protein
Target Symbol IL7
Alternative Names Interleukin 7, IL7
Accession Number P13232
Expression System E.Coli.
Molecular Weight 17.5 kDa (predicted)
Sequence Asp26-His177, with an N-terminal Met
Purity >98% by SDS-PAGE and quantitative densitometry by CoomassiePurity ® Blue staining
Activity Determined by a cell proliferation assay using 2E8 mouse cells. The ED50 for this effect is typically <0.75 ng/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.05 EU per 1 μg of the protein as determined by the LAL method
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS. If desired, 5% Trehalose or 0.1% BSA may be added.
Target Function Interleukin 7 (IL-7) functions in regulating hematopoeitic stem cell differentiation into lymphoid progenitor cells. IL-7 also plays a role in the proliferation and survival of B cells, T cells and NK cells in the lymphoid lineage. This is led to further developments in applying IL-7 in cell culture and manufacturing strategies in cancer immunotherapy approaches, such as adoptive cell therapy (ACT) and chimeric antigen receptor (CAR) T cell therapy.
Tissue Specificity IL-7 is primarily produced by stromal cells in the bone marrow and thymus with expression also reported in lymph nodes, spleen, intestinal goblet cells, keratinocytes, lung epithelium and liver sinusoidal endothelial cells.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of IL-7 has been implicated in autoimmune diseases (rheumatoid arthritis, multiple sclerosis, Crohn’s disease, ulcerative colitis), aplastic anemia myelodysplastic syndromes, asthma and chronic obstructive pulmonary disease (COPD), Graft-versus-Host Disease (GVHD), and hematological malignancies (acute lymphoblastic leukemia, T cell lymphoma).

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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