Recombinant Human IL-21 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0022UL
Recombinant Human IL-21 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 16 kDa and purity greater than 98%.
Recombinant Human IL-21 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 16 kDa and purity greater than 98%.

Recombinant Human IL-21 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0022UL
/
Size

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Product Overview

Product Name Recombinant Human IL-21 Protein
Target Symbol IL21
Alternative Names Interleukin 21, Za11, CVID11
Accession Number Q9HBE4
Expression System E.Coli.
Molecular Weight 15.4 kDa (predicted)
Sequence Gln32-Ser162, with an N-terminal Met
Purity >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Measured by a dose-dependent induction of human IFN-gamma secretion by human natural killer lymphoma cells. The ED50 for this effect is typically <7 ng/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.01 EU/1ug of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Interleukin-21 (IL-21) is a cytokine that plays a role in coordinating immune responses and maintaining immune system balance. IL-21 is produced by NKT cells, Th17 cells and T follicular helper cells (Tfh). It functions in downregulating regulatory T (Treg) cells and modulating B cell activation and differentiation into plasma cells and memory B cells. Through the stimulation of NK cells and CD8 T cells, IL-21 is associated with antitumor activity. IL-21 signaling occurs through the heterodimer receptor complex of IL-21R (CD360) and the common gamma chain (CD132).
Tissue Specificity IL-21 expression has been identified in spleen, lymph nodes, tonsils, thymus, gut-associated lymphoid tissue (GALT), mucosa-associated lymphoid tissue (MALT), skin, and liver.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of IL-21 is associated with autoimmune disorders (rheumatoid arthritis, systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD)), asthma, allergic rhinitis, psoriasis, and atopic dermatitis.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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