Recombinant Human IgJ Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2607
Recombinant Human IgJ Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2607
Catalog No.: BLPSN-2607
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_653247.1 |
| Synonym | IGCJ, JCH |
| Background | Immunoglobulin J chain, also known as IGJ and IGCJ, is a secreted polypeptide which is the first immunoglobulin-related polypeptide expressed during the embryogenesis and differentiation of B cells in the fetal liver. The joining Immunoglobulin J chain is a small polypeptide, expressed by mucosal and glandular plasma cells, which regulates polymer formation of immunoglobulin (Ig)A and IgM. Immunoglobulin J chain / IGJ serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. Immunoglobulin J chain / IGJ also help to bind these immunoglobulins to secretory component. J-chain incorporation into polymeric IgA (pIgA, mainly dimers) and pentameric IgM endows these antibodies with several salient features. Immunoglobulin J chain / IGJ is involved in creating the binding site for pIgR / SC in the Ig polymers, not only by determining the polymeric quaternary structure but apparently also by interacting directly with the receptor protein. Both the immunoglobulin J chain / IGJ and the pIgR/SC are key proteins in secretory immunity. |
| Description | A DNA sequence encoding the mature form of human IGJ (NP_653247.1) (Gln 23-Asp 159) was fused with a His tag at the C-terminus and a pelB singnal peptide at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Gln 23 |
| AA Sequence | Gln 23-Asp 159 |
| Molecular Weight | The recombinant human IGJ comprises 147 a.a. and has a predicted molecular mass of 17 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
