Recombinant Human IFN-gamma Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0021UL
Recombinant Human IFN-gamma (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 16 kDa and purity greater than 97%.
Recombinant Human IFN-gamma (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 16 kDa and purity greater than 97%.

Recombinant Human IFN-gamma Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0021UL
/
Size

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Product Overview

Product Name Recombinant Human IFN-gamma Protein
Target Symbol IFNG
Alternative Names Interferon gamma, IFNG, Immune Interferon, IFG, IFI
Accession Number CAA31639
Expression System E.Coli.
Molecular Weight 16.9 kDa (predicted)
Sequence Gln24-Gln166, with an N-terminal Met
Purity >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by the ability to inhibit proliferation of HT-29 human cells. The ED50 for this effect is typically <0.75 ng/mL
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.005 EU per 1 µg of the protein as determined by the LAL method
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile, deionized water.
Target Function Interferon-gamma (IFN-gamma) is a protein with predominant roles in the innate and adaptive immune response. It is secreted by Th1 cells, cytotoxic T cells, macrophages, dendritic cells, mucosal epithelial cells and NK cells. IFN-gamma promotes the differentiation of T cells into Th1 cells. Th1 cells produce IFN-gamma themselves, creating a positive feedback loop that amplifies the immune response. In addition, IFN-gamma upregulates monocytes, macrophages, and dendritic cells while increasing MHC class I and II expression. B Cell immunoglobulin class switching is also influenced by IFN-gamma. IFN-gamma RI (CD119/IFNGR1) is the ligand-binding subunit that heterodimerizes with the IFN-gamma RII (IFNGR2).
Tissue Specificity IFN-gamma expression has been identified in vascular endothelial cells, respiratory and gastrointestinal epithelial cells.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of IFN-gamma is associated with autoimmune disorders (multiple sclerosis (MS), rheumatoid arthritis (RA), type 1 diabetes, and inflammatory bowel diseases (IBD)), allergic asthma, atherosclerosis, hemophagocytic lymphohistiocytosis (HLH), Alzheimer’s disease, Parkinson’s disease and multiple sclerosis (MS).

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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