Recombinant Human IFI30 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2519
Recombinant Human IFI30 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2519
Catalog No.: BLPSN-2519
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P13284 |
| Synonym | GILT, IFI-30, IFI30, IP-30, IP30 |
| Background | IFI3 belongs to the GILT family. This family includes the two characterised human gamma-interferon-inducible lysosomal thiol reductase (GILT) sequences: P13284 and Q9UL8. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. In addition, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulphide bond reduction. IFI3 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. It also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. IFI3 may facilitate the complete unfolding of proteins destined for lysosomal degradation and plays an important role in antigen processing. |
| Description | A DNA sequence encoding the human IFI30 (P13284) (Met1-Lys243) with a C-terminal His tag was expressed. |
| Source | HEK293 |
| Predicted N Terminal | Ser 27 |
| AA Sequence | Met1-Lys243 |
| Molecular Weight | The recombinant human IFI30 is a disulfide-linked homodimer. The reduced monomer comprises 217 a.a. and has a predicted molecular mass of 24.7 kDa. The apparent molecular mass of the protein is approximately 31-34 kDa in SDS-PAGE under reducing conditions. |
| Purity | >96% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
