Recombinant Human ICAM2 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2491
Recombinant Human ICAM2 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2491
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His&Fc |
Host Species | Human |
Accession | NP_000864.2 |
Synonym | CD102 |
Background | Intercellular adhesion molecule 2 (ICAM-2, CD12), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment. |
Description | A DNA sequence encoding the extracellular domain (Met 1-Gln 223) of human ICAM2 (NP_000864.2) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Ser 22 |
AA Sequence | Met 1-Gln 223 |
Molecular Weight | The recombinant human ICAM2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 449 a.a. and has a predicted molecular mass of 50.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhICAM2/Fc monomer is approximately 85-95 kDa due to glycosylation. |
Purity | >97% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When cells are added to ICAM2-coated plates (12.5 ug/mL, 100 uL/well), approximately 50 % - 60 % will adhere specifically. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |