Recombinant Human ICAM1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2485
Recombinant Human ICAM1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2485
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_000192.2 |
| Synonym | BB2, CD54, ICAM-1, P3.58 |
| Background | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 9 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
| Description | A DNA sequence encoding the human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) was fused with the a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Gln 28 |
| AA Sequence | Met 1-Glu 480 |
| Molecular Weight | The recombinant human ICAM1 consists of 464 a.a. and has a predicted molecular mass of 51 kDa. As a result of glycosylation, the apparent molecular mass of rhICAM1 is approxiamtely 65-70 kDa in SDS-PAGE under reducing conditions. |
| Purity | >98% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM1-coated plates (12.5 ug/ml, 100 ul/well), approximately 30%-60% will adhere specifically. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
