Recombinant Human HSPB3 Protein

Beta LifeScience SKU/CAT #: BL-3300PS
Recombinant Human HSPB3 Protein

Recombinant Human HSPB3 Protein

Beta LifeScience SKU/CAT #: BL-3300PS
Catalog No.: BL-3300PS

Product Overview

Tag N/A
Host Species Human
Synonym Heat shock 27kDa protein 3, HSPL27, Protein 3, HSP 17, DHMN2C, HMN2C, heat shock protein beta-3, HspB3.
Background HSPB3 is a muscle-specific small heat shock protein which is expressed specially in heart and skeletal muscle. Mammalian small heat shock proteins (sHSP) are highly expressed proteins with varied roles, including the assembly and impounding of multi-protein complexes, transportation of nascent polypeptide chains thru cellular membranes and regulation of protein folding. HSPB3 perform as an ATP-dependent chaperone protein which takes part in the refolding of denatured proteins and inhibition of actin polymerization.
Description HSPB3 Human Recombinant expressed in E.coli is a single, non-glycosylated polypeptide chain containing 170a.a. (1-150) and having a molecular weight of 19.1 kDa.The HSPB3 is fused to a 20a.a. His-Tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MAKIILRHLI EIPVRYQEEF EARGLEDCRL DHALYALPGP TIVDLRKTRA AQSPPVDSAA ETPPREGKSH FQILLDVVQF LPEDIIIQTF EGWLLIKAQH GTRMDEHGFI SRSFTRQYKL PDGVEIKDLS AVLCHDGILV VEVKDPVGTK
Purity >85% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The HSPB3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1M Urea and 5% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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