Recombinant Human HIPK2 Protein

Beta LifeScience SKU/CAT #: BL-0334SG
Recombinant Human HIPK2 Protein

Recombinant Human HIPK2 Protein

Beta LifeScience SKU/CAT #: BL-0334SG
Catalog No.: BL-0334SG

Product Overview

Tag GST
Host Species Human
Accession NM_022740
Synonym DKFZp686K02111; FLJ23711; PRO0593
Background HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors. HIPK2 enhances expression of p53 target genes that resulting in growth arrest and the enhancement of UV-induced apoptosis that could be inhibited by antisense to HIPK2 (1). HIPK2 as a target for MDM2 mediated ubiquitin-dependent degradation that only occurred in growth-arresting conditions when MDM2 was efficiently induced by p53 (2). The knockdown of HIPK2 using small interfering RNA reduced p53 binding and activation of p53R2 that resulting in impaired UV-induced DNA repair.
Description Recombinant human HIPK2 (1-640) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source Sf9 insect cells
AA Sequence 1-640
Molecular Weight ~98 kDa
Purity For specific purity information on a given lot, see related COA.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Active
Formulation Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability The recombinant protein is stable for up to 12 months at -70°C
Usage For Research Use Only
Storage Recombinant Human HIPK2 Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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