Recombinant Human HGK Protein
Beta LifeScience
SKU/CAT #: BL-0285SG
Recombinant Human HGK Protein
Beta LifeScience
SKU/CAT #: BL-0285SG
Catalog No.: BL-0285SG
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | NP_004825 |
| Synonym | MAP4K4, NIK, FLH21957, FLJ10410, FLJ20373, FLJ90111, KIAA0687 |
| Background | HGK is the mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) and a member of the serine/threonine protein kinase family. HGK has been shown to specifically activate MAPK8/JNK (1). The activation of MAPK8 by HGK can be inhibited by dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggest that this kinase functions through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediates TNF-alpha signaling. HGK-dependent signaling inhibits PPARγ-responsive gene expression, adipogenesis, and insulin-stimulated glucose transport (2). |
| Description | Recombinant human HGK (1-328) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. |
| Source | Sf9 insect cells |
| AA Sequence | 1-328 |
| Molecular Weight | ~64 kDa |
| Purity | For specific purity information on a given lot, see related COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Active |
| Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
| Stability | The recombinant protein is stable for up to 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Recombinant Human HGK Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
