Recombinant Human HGK Protein

Beta LifeScience SKU/CAT #: BL-0285SG
Recombinant Human HGK Protein

Recombinant Human HGK Protein

Beta LifeScience SKU/CAT #: BL-0285SG
Catalog No.: BL-0285SG

Product Overview

Tag GST
Host Species Human
Accession NP_004825
Synonym MAP4K4, NIK, FLH21957, FLJ10410, FLJ20373, FLJ90111, KIAA0687
Background HGK is the mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) and a member of the serine/threonine protein kinase family. HGK has been shown to specifically activate MAPK8/JNK (1). The activation of MAPK8 by HGK can be inhibited by dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggest that this kinase functions through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediates TNF-alpha signaling. HGK-dependent signaling inhibits PPARγ-responsive gene expression, adipogenesis, and insulin-stimulated glucose transport (2).
Description Recombinant human HGK (1-328) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source Sf9 insect cells
AA Sequence 1-328
Molecular Weight ~64 kDa
Purity For specific purity information on a given lot, see related COA.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Active
Formulation Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability The recombinant protein is stable for up to 12 months at -70°C
Usage For Research Use Only
Storage Recombinant Human HGK Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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