Recombinant Human HER3 / ERBB3 Protein (aa 730-1065, His&GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2411
Recombinant Human HER3 / ERBB3 Protein (aa 730-1065, His&GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2411
Catalog No.: BLPSN-2411
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | NP_001973.2 |
| Synonym | ERBB3, ErbB-3, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, erb-b2 receptor tyrosine kinase 3, FERLK |
| Background | Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene.ErbB3 is a member of the epidermal growth factor receptor (EGFR/ERBB) family of receptor tyrosine kinases. The kinase-impaired ErbB3 is known to form active heterodimers with other members of the ErbB family, most notably the ligand binding-impaired ErbB2.ERBB3 (Erb-B2 Receptor Tyrosine Kinase 3) is a Protein Coding gene. Diseases associated with ERBB3 include Lethal Congenital Contracture Syndrome 2 and Erythroleukemia, Familial. Among its related pathways are PI3K/AKT activation and RET signaling. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is ERBB4. |
| Description | A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Pro 730-Ser 1065 |
| Molecular Weight | The recombinant human ErbB3 /GST chimera consists of 573 amino acids and has a calculated molecular mass of 65 KDa. It migrates as an approximately 65 KDa band as predicted in SDS-PAGE under reducing conditions. |
| Purity | >75% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | 1. No Kinase Activity2. Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST bound to human NRG1 (aa 2-224) was 20 nM. |
| Formulation | Recombinant Human HER3 / ERBB3 Protein (aa 730-1065, His&GST Tag) was lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.5, 10% gly, 1mM GSH |
| Stability | Recombinant Human HER3 / ERBB3 protein is stable up to 1 year at -70°C |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
