Recombinant Human HDAC8 Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2376
Recombinant Human HDAC8 Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2376
Catalog No.: BLPSN-2376
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | NP_060956.1 |
| Synonym | CDA07, CDLS5, HD8, HDACL1, MRXS6, RPD3, WTS |
| Background | Histone deacetylase 8, also known as HDAC8 and HDACL1, is a nucleus and cytoplasm protein which belongs to thehistone deacetylase family and HD type 1 subfamily. Histone deacetylases (HDACs) are a growing family of enzymes implicated in transcriptional regulation by affecting the acetylation state of core histones in the nucleus of cells.HDAC8 / HDACL1 is weakly expressed in most tissues. It expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. HDAC8 / HDACL1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones ( H2A, H2B, H3 and H4 ). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC8 / HDACL1 may play a role in smooth muscle cell contractility. HDAC8 / HDACL1 may be a potential drug target for neuroblastoma differentiation therapy using selective inhibitors, avoiding unspecific side effects. |
| Description | A DNA sequence encoding the full length of human HDAC8 isoform 1 (NP_060956.1) (Met 1-Val 377) was expressed, fused with the GST tag at the C-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Val 377 |
| Molecular Weight | The recombinant human HDAC8/GST chimera consists of 602 a.a. and has a predicted a molecular mass of 68 kDa as estimated in SDS-PAGE under reducing conditions. |
| Purity | >88% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
