Name: Recombinant Human HAI-2 Protein (ECD, Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-2364
Availability: InStock

Recombinant Human HAI-2 Protein (ECD, Fc Tag)

Collections: Other recombinant proteins, Recombinant proteins

Product Overview

Tag	Fc
Host Species	Human
Accession	NP_066925.1
Synonym	DIAR3, HAI-2, HAI2, Kop, PB
Background	Choanal (CA) and gastrointestinal atresias (GA) are an important feature of syndromic congenital sodium diarrhea (sCSD), a disorder recently associated with mutations in the gene for serine protease inhibitor type 2 (SPINT2). The SPINT2 gene is epigenetically silenced or downregulated in human cancers, altering the balance of HGF activation/inhibition ratio, which contributes to cancer development and progression. SPINT2 is a tumor suppressor gene that inhibits proteases implicated in cancer progression, like HGFA, hepsin and matriptase. Loss of SPINT2 expression in tumors has been associated with gene promoter hypermethylation. SPINT2 (serine peptidase inhibitor Kunitz type 2), a proteolytic inhibitor of hepatocyte growth factor activator (HGFA), which has a significant role in the suppression of the HGF-MET pathway and malignant melanoma progression.
Description	A DNA sequence encoding the human SPINT2 (NP_066925.1) (Met1-Lys197) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Ala 28
AA Sequence	Met1-Lys197
Molecular Weight	The recombinant human SPINT2 consists 408 a.a. and predicts a molecular mass of 45.9 kDa.
Purity	>90% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Bioactivity	Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2.The IC50 value is < 1 nM.
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.