Recombinant Human GNGT1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2295
Recombinant Human GNGT1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2295
Catalog No.: BLPSN-2295
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P63211 |
| Synonym | GNG1 |
| Background | GNGT1 is a subunit of of transducin. Heterotrimeric G proteins consist of alpha, beta, and gamma subunits. They are membrane bound GTPases that are linked to 7-TM receptors. They function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. They are involved as a modulator or transducer in various transmembrane signaling systems. G proteins are bound to GDP in the 'off' state. GNGT1 is the gamma subunit of transducin. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; in addition, some Gbetagamma subunits have active functions. Gbetagamma coupled to H1 receptors can activate PLA2 and Gbetagamma coupled to M1 receptors can activate KIR channels. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. |
| Description | A DNA sequence encoding the mature foem of human GNGT1 (P63211) (Pro 2-Cys 71) was expressed, with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Pro 2-Cys 71 |
| Molecular Weight | The recombinant human GNGT1 consisting of 85 a.a. and has a calculated molecular mass of 9.9KDa. It migrates as a 9.0 kDa band in SDS-PAGE under reducing conditions as predicted. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.5. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
