Recombinant Human GFR alpha-1 / GDNFR Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2234
Recombinant Human GFR alpha-1 / GDNFR Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2234
Collections: Other recombinant proteins, Recombinant proteins
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Product Overview
Tag | His |
Host Species | Human |
Accession | NP_665736.1 |
Synonym | GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1 |
Background | Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-beta (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells. |
Description | A DNA sequence encoding the human GFRalpha1 without the propeptide (NP_665736.1) (Met 1-Ser 424) was fused with the a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Asp 25 |
AA Sequence | Met 1-Ser 424 |
Molecular Weight | The recombinant human GFRalpha1 consists of 411 a.a. after removal of the signal peptide and predicts a molecular mass of 46 kDa. As a result of glycosylation, the rhGFRalpha1 migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions. |
Purity | >98% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.2-1 ug/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind human GDNF (native) in functional ELISA. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. |
Subcellular Location | Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus, trans-Golgi network. Endosome. Endosome, multivesicular body. |
Protein Families | GDNFR family |
Database References |
Gene Functions References
- circGFRA1 may function as a competing endogenous RNA (ceRNA) to regulate GFRA1 expression through sponging miR-34a to exert regulatory functions in TNBC. PMID: 29037220
- the methylation status of CpG sites in GFRA1 and GSTM2 may have a role and could be used as potential biomarkers for the screening of rectal cancer PMID: 27566576
- GFRA1 regulates AMPK-dependent autophagy by promoting SRC phosphorylation independent of proto-oncogene RET kinase PMID: 27754745
- that the consequences of this is that GFRalpha-1-mediated signalling is altered during the ageing process PMID: 27346872
- RET c.1296A may be a common susceptibility allele for nephron underdosing-related diseases. The 5'-UTR and intronic variants near exon 5 of GFRA1 are not associated with nephron endowment. PMID: 27533506
- Hox proteins coordinate motor neuron differentiation and connectivity programs through Ret/Gfra genes. PMID: 26904955
- This study demonstrate, using a knock-in mouse model in which GFRalpha1 is no longer located in lipid rafts, that the developmental functions of GDNF in the periphery require the translocation of the GDNF receptor complex into lipid rafts. PMID: 26400951
- Methylation changes of GFRA1, SRF, and ZNF382 may be a potential biomarker set for prediction of gastric carcinoma metastasis. PMID: 25009298
- GFRalpha1 levels in neurons from autopsied AD brains are significantly decreased. PMID: 25253858
- In the cochlea, GFRalpha-1 was identified mainly in the cell bodies of the spiral neurons. In the organ of Corti, GFRalpha-1 was demonstrated in the Deiters' cells, Hensen cells, inner pillar cells, and weakly in inner hair cells but not in the outer hair cells. PMID: 24139947
- The study shows co-localization of RET with GFRA1 and GFRA2 in myenteric ganglia of the adult human colon. PMID: 23881409
- The expression of GFRalpha1 and/or GFRalpha3, especially when combined with ARTN expression, may be useful predictors of disease progression and outcome in specific subtypes of mammary carcinoma. PMID: 23351331
- Mutations in GFRA1 gene is associated with urinary tract malformations. PMID: 22729463
- Post-synaptic transgenic GFRalpha1 has profound effects on the development of dopamine neurons, resulting in a 40% increase in the adult number. PMID: 21133924
- This study found nominally-significant evidence for interactions between GFRA1, 2 and 3 associated with schizophrenia and clozapine response, consistent with the locations of these three genes within linkage regions for schizophrenia. PMID: 20116071
- Results identify persephin, a GDNF family member, as a novel ligand for GFRalpha1/RET receptor complex. PMID: 20350599
- MEN2 arises from activating missense mutations in RET, causing autodimerization under certain conditions. The position of missense mutations influences medullary thyroid carcinoma aggressiveness. Review. PMID: 20669561
- Here we report that human nigral dopaminergic neurons express GFRalpha1 and RET receptors at all ages. There was no reduction in the number of neurons expressing these receptors as a function of age. PMID: 20347960
- GFR-alpha1 mRNA transcripts were detected in oocytes and GCs from all samples from fetuses, girls and adult women. PMID: 19896648
- The expression of GFRA1 in normal infants and normoganglionic colon of patients with Hirschsprung's disease was restricted to receptor tyrosine kinase(RET)-negative glial cells and RET-positive neurons of the ganglionic plexus. PMID: 12065680
- GFRA1-193C > G and 537T > C could be in linkage disequilibrium with other loci responsible for medullary thyroid cancer PMID: 12490080
- analysis of binding surface for the GDNF-GFR alpha 1 PMID: 14514671
- GFRalpha-1 were observed within sensory and motor nuclei of cranial nerves, dorsal column nuclei, olivary nuclear complex, reticular formation, pontine nuclei, locus caeruleus, raphe nuclei, substantia nigra, and quadrigeminal plate. PMID: 17825269
- GDNF can act as an important component of the inflammatory response in breast cancers and its effects aare mediated by GFR alpha 1 receptors. PMID: 18089803
- GDNF is a key component to preserve several cell populations in the nervous system and also participates in the survival and differentiation of peripheral neurons. PMID: 18394855
- 38 cases of germ cell tumors: 26 cases contained immature teratoma, of which 24 had immature neuroepithelium and showed strong membrane staining for GFRalpha-1. staining for GFRalpha-1 in immature neuroepithelium may facilitate its identification. PMID: 19019765