Recombinant Human Geranylgeranyl Pyrophosphate Synthase (GGPS1) Protein (His-SUMO)

Name: Recombinant Human Geranylgeranyl Pyrophosphate Synthase (GGPS1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-09811P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Geranylgeranyl Pyrophosphate Synthase (GGPS1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O95749
Target Symbol	GGPS1
Synonyms	(2E; 6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase 1; Geranylgeranyl diphosphate synthase; Geranylgeranyl pyrophosphate synthase; Geranylgeranyl pyrophosphate synthetase; Geranyltranstransferase; GGPP synthase; GGPP synthetase; GGPPS; GGPPS_HUMAN; GGPPS1; GGPPSase; GGPS1; OTTHUMP00000036073; OTTHUMP00000216161; OTTHUMP00000216163; OTTHUMP00000216164
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MEKTQETVQRILLEPYKYLLQLPGKQVRTKLSQAFNHWLKVPEDKLQIIIEVTEMLHNASLLIDDIEDNSKLRRGFPVAHSIYGIPSVINSANYVYFLGLEKVLTLDHPDAVKLFTRQLLELHQGQGLDIYWRDNYTCPTEEEYKAMVLQKTGGLFGLAVGLMQLFSDYKEDLKPLLNTLGLFFQIRDDYANLHSKEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTENIDIKKYCVHYLEDVGSFEYTRNTLKELEAKAYKQIDARGGNPELVALVKHLSKMFKEENE
Expression Range	1-300aa
Protein Length	Full Length
Mol. Weight	50.9kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate, an important precursor of carotenoids and geranylated proteins.
Subcellular Location	Cytoplasm.
Protein Families	FPP/GGPP synthase family
Database References	HGNC: 4249 OMIM: 606982 KEGG: hsa:9453 STRING: 9606.ENSP00000282841 UniGene: Hs.730768
Tissue Specificity	Abundantly expressed in testis. Found in other tissues to a lower extent.

Gene Functions References

results may support a model in which accumulation of susceptibility variants (including some in relevant genes, notably GGPS1) may lead to a possible genetic component of predisposition to atypical femoral fractures. PMID: 28467865
GGPPS1 may play a critical role during the development of HCC from cirrhosis and is of clinical significance for predicting biological character of HCC. PMID: 24716791
Egr-1 most likely does not induce the constitutive activation of Erk1/2 through its target gene GGPS1. PMID: 23478574
the Egr-1/GGPPS/Erk1/2 pathway is responsible for insulin resistance during hyperinsulinism PMID: 21321112
these results reveal a new EGR-1/GGPPS/MAPK signaling pathway that controls cigarette smoke-induced pulmonary inflammation. PMID: 21224049
Study suggested that GGPS1 -8188A ins/del polymorphism may confer susceptibility to femoral neck BMD response to bisphosphonate therapy in Korean women. PMID: 20191015
crystal structure; it reveals that three dimers join together to form a propeller-bladed hexameric molecule with a mass of approximately 200 kDa PMID: 16698791
Farnesyltranstransferase mutations are associated with lonafarnib resistance PMID: 17536018
results indicate that the active form of GGPS in the solution is an octamer rather than hexamer or dimer PMID: 17646172
Evidence for a non-membrane bound isoform of geranylgeranyl diphosphate synthase 1 in rat. PMID: 8419360

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.