Recombinant Human General Transcription Factor Iif Subunit 2 (GTF2F2) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-10000P

Greater than 90% as determined by SDS-PAGE.
Recombinant Human General Transcription Factor Iif Subunit 2 (GTF2F2) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-10000P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human General Transcription Factor Iif Subunit 2 (GTF2F2) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | P13984 |
Target Symbol | GTF2F2 |
Synonyms | ATP dependent helicase GTF2F2; ATP-dependent helicase GTF2F2; BTF4; General transcription factor IIF 30 kDa subunit; General transcription factor IIF polypeptide 2; General transcription factor IIF subunit 2; General transcription factor IIF; polypeptide 2; 30kDa; Gtf2f2; RAP30; T2FB_HUMAN; TF2F2; TFIIF; TFIIF beta; TFIIF-beta; Transcription initiation factor IIF subunit beta; Transcription initiation factor RAP30 |
Species | Homo sapiens (Human) |
Expression System | E.coli |
Tag | N-6His-SUMO |
Target Protein Sequence | AERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNEDLANIHDIGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAASENYMRLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEKSD |
Expression Range | 2-249aa |
Protein Length | Full Length of Mature Protein |
Mol. Weight | 44.2kDa |
Research Area | Transcription |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity. |
Subcellular Location | Nucleus. |
Protein Families | TFIIF beta subunit family |
Database References |
Gene Functions References
- Results pinpoint critical residues for RNA polymerase II subunit 5 binding to RAP30 and/or to HBx, and identify these residues in both mammalian cells and in an in vitro binding assay. PMID: 16169872