Recombinant Human GDNF Protein

Beta LifeScience SKU/CAT #: BLPSN-2226
Recombinant Human GDNF Protein

Recombinant Human GDNF Protein

Beta LifeScience SKU/CAT #: BLPSN-2226
Catalog No.: BLPSN-2226

Product Overview

Tag N/A
Host Species Human
Accession P39905
Synonym ATF1, ATF2, HFB1-GDNF, HSCR3
Background Glial cell line-derived neurotrophic factor(GDNF) is an important member of the GDNF family of ligands(GFL). The GDNF family of ligands is comprised by four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN). It has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. As the founding member, GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. It has been shown that GDNF results in two Parkinson's disease clinical trial and in a number of animal trials. It has been taken as a potent survival factor for central motoneurons.
Description A DNA sequence encoding the mature form of human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.
Source HEK293
Predicted N Terminal Gly
AA Sequence Arg 83-Ile 185
Molecular Weight The recombinant human GDNF consists of 105 a.a. and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.
Purity >97% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-15 ng/mL in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 His Chimera.2. Measured by its ability to bind biotinylated Human GFRa1-His in functional ELISA.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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