Recombinant Human GDNF Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0011
Recombinant Human GDNF (2 ug/lane) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained using Coomassie® Blue showing single bands at 16 kDa and 32 kDa, respectively, and purity greater than 97%.
Recombinant Human GDNF (2 ug/lane) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained using Coomassie® Blue showing single bands at 16 kDa and 32 kDa, respectively, and purity greater than 97%.

Recombinant Human GDNF Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0011
/
Size

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Product Overview

Product Name Recombinant Human GDNF Protein
Target Symbol GDNF
Alternative Names Glial Cell Line Derived Growth Factor, Glial Derived Neurotrophic Factor, Astrocyte-Derived Trophic Factor, HFB1-GDNF, ATF, ATF1, ATF2, HSCR3, HGDNF
Accession Number P39905
Expression System CHO cell line. Disulfide-linked homodimer.
Molecular Weight 11.6 kDa (monomer, predicted), 16 kDa and 32 kDa (glycosylated, observed on SDS-PAGE gel stained using Coomassie® Blue under reducing and non-reducing conditions, respectively)
Sequence Arg109-Ile211
Purity >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining.
Activity Determined by a cell proliferation assay using human neuroblastoma cells. The ED50 for this effect is typically <20 ng/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.05 EU/1ug of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Glial Cell-Derived Neurotrophic Factor (GDNF) plays multiple roles in neuronal development, maintenance, and repair. GDNF is produced by astrocytes, oligodendrocytes, Schwann cells, motor neurons, and skeletal muscle to regulate the survival of motor neurons and differentiation dopaminergic neurons during development and to protect against subsequent neurodegeneration. GDNF production in Sertoli cells regulates male germ cell proliferation and differentiation. In cell culture, GDNF is often used to differentiate human pluripotent stem cells (hPSC) progenitors into astrocytes and neurons. GDNF is also used to maintain survival and growth of neurons and astrocytes in culture. Under further culture conditions, GDNF is able to contribute to the maintenance and differentiation of dopaminergic neurons. GDNF signaling takes places by binding to the receptor, GFR alpha1, and forming a complex with RET.
Tissue Specificity GDNF expression has been identified in brain (astrocytes, neurons, substantia nigra), kidney, skeletal muscle, testes, and colon.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of GDNF is associated with Parkinson’s disease, amyotrophic lateral sclerosis (ALS), Huntington’s disease, depression, and drug and alcohol addiction.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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