Recombinant Human GCAP1 Protein
Beta LifeScience
SKU/CAT #: BLPSN-2211
Recombinant Human GCAP1 Protein
Beta LifeScience
SKU/CAT #: BLPSN-2211
Catalog No.: BLPSN-2211
Product Overview
| Tag | N/A |
| Host Species | Human |
| Accession | P43080 |
| Synonym | C6orf131, COD3, CORD14, dJ139D8.6, GCAP, GCAP1, GUCA, GUCA1 |
| Background | GCAP 1 gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase activating protein 1 (GCAP 1), mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP 1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in GCAP 1 gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. GCAP 1 stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure. |
| Description | A DNA sequence encoding the human GUCA1A (P43080)(Met1-Gly201) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Gly |
| AA Sequence | Met1-Gly201 |
| Molecular Weight | The secreted recombinant human GUCA1A consists of 203 a.a. and predicts a molecular mass of 23.1 KDa. The apparent molecular mass of the protein is approximately 20 KDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
