Recombinant Human GAD67 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2179
Recombinant Human GAD67 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2179
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | Q99259 |
| Synonym | CPSQ1, GAD, SCP |
| Background | Glutamate decarboxylase 1, also known as 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform and GAD1, is a member of thegroup II decarboxylase family. GAD1 is expressed in benign and malignant prostatic tissue and may serve as a highly prostate-specific tissue biomarker. GAD1 isoform3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Tissue-specific markers are useful for identification of tumour type in advanced cancers of unknown origin. In plants, as in most eukaryotes, glutamate decarboxylase catalyses the synthesis of GABA. Root-specific calcium/calmodulin-regulated GAD1 plays a major role in GABA synthesis in plants under normal growth conditions and in response to stress. Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1)which is a non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. |
| Description | A DNA sequence encoding the human GAD1 (Q99259-1) (Met 1-Leu 594) was fused with a His tag at the C-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met 1 |
| AA Sequence | Met 1-Leu 594 |
| Molecular Weight | The recombinant human GAD1 consists of 605 a.a. and predicts a molecular mass of 68.3 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions. |
| Purity | >92% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.5. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
