Name: Recombinant Human Frizzled 5 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2150
Availability: InStock

Recombinant Human Frizzled 5 Protein (His Tag)

Catalog No.: BLPSN-2150

Product Overview

Tag	His
Host Species	Human
Accession	NP_003459.2
Synonym	C2orf31, HFZ5
Background	Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 1 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt1B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.
Description	A DNA sequence encoding the extracellular domain (Met 1-Pro 167) of human frizzled 5 (NP_003459.2) was fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Ala 27
AA Sequence	Met 1-Pro 167
Molecular Weight	The recombinant human frizzled5 consists of 152 a.a. and has a predicted molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhfrizzled5 is approximately 25-30 kDa due to glycosylation.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.