Recombinant Human Frizzled 5 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-2150
Recombinant Human Frizzled 5 Protein (His Tag)

Recombinant Human Frizzled 5 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-2150
Catalog No.: BLPSN-2150

Product Overview

Tag His
Host Species Human
Accession NP_003459.2
Synonym C2orf31, HFZ5
Background Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 1 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt1B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.
Description A DNA sequence encoding the extracellular domain (Met 1-Pro 167) of human frizzled 5 (NP_003459.2) was fused with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Ala 27
AA Sequence Met 1-Pro 167
Molecular Weight The recombinant human frizzled5 consists of 152 a.a. and has a predicted molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhfrizzled5 is approximately 25-30 kDa due to glycosylation.
Purity >97% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed