Recombinant Human FOLR2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2130
Recombinant Human FOLR2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2130
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | P14207 |
Synonym | BETA-HFR, FBP, FBP/PL-1, FR-BETA, FR-P3 |
Background | Folate receptor beta, also known as Folate receptor 2, FBP, and FOLR2, is a member of the folate receptor family. FOLR2 is expressed in placenta and hematopoietic cells. The expression of FOLR2 is increased in malignant tissues. Members of the Folate receptor family members (FOLRs) have a high affinity for folic acid and for several reduced folic acid derivatives. They mediate the delivery of 5-methyltetrahydrofolate to the interior of, out of within, or between cells in a process known as potocytosis. FOLR2 has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein was originally thought to exist only in placenta, but is also detected in spleen, bone marrow, and thymus. FOLR2 is a marker for macrophages generated in the presence of M-CSF, but not GM-CSF. Its expression correlates with increased folate uptake ability. Folate conjugates of therapeutic drugs are a potential immunotherapy tool to target tumor-associated macrophages. |
Description | A DNA sequence encoding the human FOLR2 (P14207-1) without the propeptide (Met 1-His 228) was fused with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Thr 17 |
AA Sequence | Met 1-His 228 |
Molecular Weight | The recombinant human FOLR2 consists of 223 a.a. and predictes a molecular mass of 26 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh FOLR2 is approximately 30-35 kDa due to glycosylation. |
Purity | >87% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. |
Subcellular Location | Cell membrane; Lipid-anchor, GPI-anchor. Secreted. |
Protein Families | Folate receptor family |
Database References | |
Tissue Specificity | Expressed in placenta and hematopoietic cells. Expression is increased in malignant tissues. |
Gene Functions References
- Folate receptor beta as a novel CD11b/CD18 regulator for trafficking and homing of a subset of macrophages on collagen. PMID: 27534550
- FR-beta expression was low or absent in the majority of ovarian, breast and colorectal tumor samples. PMID: 26248049
- FR-beta is uniquely expressed on this proinflammatory subpopulation offers a new strategy to suppress migration of inflammatory monocytes into sites of inflammation. PMID: 25015955
- High FOLR2 mRNA expression is associated with uraemic patients on hemodialysis. PMID: 23439585
- severe pre-eclampsia is associated with decreased placental expression of FR-beta and a reduction in the number of fetal macrophages (Hofbauer cells) PMID: 23480364
- Expression of folate receptor-beta on activated macrophages holds a promising potential for early diagnosis of atherosclerosis. (Review) PMID: 22094710
- High Folate Receptor beta expressing tumor-associated macrophages are associated with pancreatic cancer. PMID: 22350599
- Functional FR-beta present on osteoarthritis synovial macrophages provides a potential tool for the diagnosis and treatment of this disease. PMID: 22211358
- study describes that functional FRbeta is specifically expressed by M-CSF-polarized (M2) macrophages as well as by ex vivo isolated tumor-associated macrophages, and that tumors induce its expression in an M-CSF-dependent manner PMID: 19951991
- FR-beta gene is a target for multiple coordinate actions of nuclear receptors for ATRA directly and indirectly acting on a transcriptional complex containing activating Sp1/ets and inhibitory AP-1 proteins. PMID: 12543860